Renal Failure
Conditions
Keywords
Renal Failure, Dialysis, Biological Aging
Brief summary
The aim of this study is to investigate the impact of renal function and dialysis techniques on the percentage of senescent T lymphocytes.
Detailed description
The immunosenescence is a complex and profound remodeling of the immune system during life. It is mainly due to thymic involution and repeated antigenic stimulation. Kidney disease is associated with a decrease in adaptive immunity as evidenced by the decrease in vaccine response and increased susceptibility to infections, similar to those observed in the elderly population. However, data on aging of the immune system in chronic kidney disease remains incomplete. Furthermore, the determinants of immunosenescence are not also not known. It is possible that uremic factors help explain the phenotypic and functional changes of lymphocytes, as antigenic stimuli associated with repeated bio-compatible materials used in dialysis contact. The purpose of this study is to describe the phenotypes of the immune system of renal and analyze the determinants of these changes.
Interventions
BIOLOGICALBlood sample
3 tubes of 5 ml tubes and 4 of 7 ml for biological assays at t0.
Sponsors
Centre Hospitalier Universitaire de Besancon
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
OTHER
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 85 Years
Healthy volunteers
No
Inclusion criteria
* Patient able to understand the reason of the study * Patient not opposed to the conservation of biological samples for scientific research
Exclusion criteria
* Patient suffering from psychotic illness * Any history of immunosuppressive therapy (except steroids up to 5mg/day) * History of cancer (except skin cancer) or treated hematological malignancy * Infectious episode required hospitalization not older 3 months * Hepatitis B or C infection * HIV infection, active or inactive * For dialysis patients: renal failure on dialysis for less than 3 months and/or have benefited from two techniques for renal replacement therapy in the last 6 months
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Percentage of Cluster of Differentiation (CD) 4/8+ CD 57+ CD 28- lymphocytes (senescent lymphocytes) | 6 months | The primary outcome measure is the percentage of CD 4/8+ CD 57+ CD 28- lymphocytes by flow cytometry. The technique used is a 6 colors surface labelling of T lymphocytes to study the T cell senescent population. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Telomerase Activity of T lymphocytes | 6 months | The telomerase activity of T lymphocytes is assessed by a method of Polymerase chain reaction (PCR)-ELISA. |
| Level of phospho-histone 2AX (gH2AX) in peripheral blood T lymphocytes | 6 months | This level is obtained by flow cytometry after permeabilization and labelling of Peripheral Blood Mononuclear Cells (PBMC). |
| Proportion of Recent Thymic Emigrants (RTE) in peripheral blood T lymphocytes | 6 months | RTE T cells will be defined by flow cytometry, according to co-expression of CD 4, CD 8,CD 31 and CD 45RA |
| T-cell receptor excision circle (TREC) level in PBMC. | 6 months | TRECs study is based on a technique of quantitative PCR using DNA extracted from PBMC. |
| Telomere length in T lymphocytes | 6 months | Study of telomere length is performed after extraction of DNA from isolated T cells. The length is determined by a quantitative PCR technique relative to a reference gene. |
Countries
France
Outcome results
None listed