Cardiovascular Disease
Conditions
Keywords
Low childhood SES, Early Life Stress, Endothelial function, Flow mediated dilation, Cardiovascular disease, Subcutaneous adipose arterioles
Brief summary
Cardiovascular disease is the leading cause of death in the United States. Other studies have shown that stress, early in life, could be a risk factor for cardiovascular disease, later in life. This study will look at the effect of early life stressors on your cardiovascular health.
Detailed description
This study will have two parts involved to investigate the role that childhood SES status has on cardiovascular health. Part one: Subjects will give a blood sample which will be processed and analyzed. Flow mediated dilation (FMD) and Pulse wave velocity (PWV/PWA) will be performed at baseline and 2 hours following either a single dose oral antioxidant cocktail (1000 mg of Vitamin C, 600 IU of Vitamin E, and 600 mg of alpha-lipoic acid) or placebo on two days separated by at least 72 hours. Nitroglycerin mediated dilation (NMD, 0.4mg sub-lingual nitroglycerin spray), will be assessed at baseline. Part two: Subjects, if interested will undergo a subcutaneous gluteal/hip fat biopsy. Micro vessels will be isolated from the adipose tissue and analyzed by pressurized myography for endothelial function and nitric oxide bioavailability.
Interventions
DIETARY_SUPPLEMENTAntioxidant cocktail
Flow-Mediated Dilation will be determined at baseline and 2 hours following acute antioxidant treatment
OTHERBiopsy
Under sterile conditions, a small incision (¼ - ½ inch) by the study doctor will be made and the piece of fat will be taken out. Fat tissue was chosen for this study because it is a big source of small blood vessels which we are studying
OTHERPlacebo
Flow-Mediated Dilation will be determined at baseline and 2 hours following acute antioxidant treatment
DRUGNMD; 0.4mg sub-lingual nitroglycerin spray
Nitroglycerin mediated dilation will be measured immediately following an administered 0.4mg dose of nitroglycerin spray, sub-lingual.
Sponsors
National Heart, Lung, and Blood Institute (NHLBI)
Augusta University
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
TREATMENT
Masking
DOUBLE (Subject, Investigator)
Eligibility
Sex/Gender
ALL
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* documented childhood SES * men and premenopausal women ages 18-40 * lean/overweight/or obese with BMI\< or equal to 39kg/m2
Exclusion criteria
* cardiovascular, renal, pulmonary, hepatic, cerebral, or metabolic,disease * use medications that affect vascular tone * post menopausal women * have class 3 obesity or BMI over 40
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Acute Change in Flow mediated dilation | Change from baseline (2 hours) | Flow-Mediated Dilation will be determined at baseline and 2 hours following acute antioxidant treatment |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Acute Change in Biomarkers of oxidative stress | Change from baseline (2 hours) | Nitrite/nitrate, antioxidant capacity, and 8-isoprostane at baseline and 2 hours following acute antioxidant treatment |
| Acute Change in Arterial Stiffness | Change from baseline (2 hours) | Pulse wave velocity will be determined at baseline and 2 hours following acute antioxidant treatment |
| Acute change in Flow mediated dilation | Immediate | Nitroglycerin mediated dilation will be measured immediately following an administered 0.4mg dose of nitroglycerin spray, sub-lingual. |
Countries
United States
Outcome results
None listed