Endometriosis, Ovarian
Conditions
Brief summary
Purpose of this study is to evaluate stem cell expression (SCE) in endometriotic (E) and non endometriotic ovarian and endometrial tissues.
Detailed description
This study was conducted on 40 women at Al-Azhar University Hospitals. They are divided into two equal groups, group 1 with ovarian endometrioma and group 2 with normal ovary. Ovarian and endometrial samples were evaluated by hematoxlin & eosin for histopathology (HP) and of oct-4 for immunohistochemistry (IH) for SCE. Evaluation of IH parameters revealed that the most important factors for evaluating SC were arranged as score ( Edessy Stem Cell Score=ESS).
Interventions
PROCEDUREOvarian and endometrial samples
Ovarian and endometrial samples were evaluated by hematoxlin &eosin for histopathology (HP) and of oct-4 for immunohistochemistry (IH) for SCE
Sponsors
Al-Azhar University
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
DIAGNOSTIC
Masking
NONE
Eligibility
Sex/Gender
FEMALE
Age
20 Years to 40 Years
Healthy volunteers
No
Inclusion criteria
* 20 to 40 years and did not receive hormonal treatment for three month
Exclusion criteria
* Diabetes mellitus, cardiovascular and kidney diseases, adenomyosis, Uterine fibroid Endometrial carcinoma and Poly cystic ovary
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Edessy Stem Cell Score (ESS) | 1 week | ESS * Intensity of stem cell marker: Negative to mild =0, Moderate =1, Strong =2 * Percentage of stained cells: 0 =0, 0-50%= 1, \>50-100% =2. * Focality : None =0, Focal =1, Diffuse= 2 * Distribution: None= 0, Epithelial or mesenchymal= 1, Epithelial and mesenchymal= 2. * Localization of positive stained cell: None=0, Cytoplasmic or nuclear =1, Cytoplasmic and nuclear= 2 |
Countries
Egypt
Outcome results
None listed