Biological Availability of Vitamins and Micronutrients
Conditions
Keywords
bioavailability, postprandial period, genetic variants, single nucleotide polymorphisms, vitamin, carotenoid, lutein, lycopene, tocopherol, cholecalciferol
Brief summary
The aim was to identify minor genetic variations (single nucleotide polymorphisms) that explain the interindividual variability in bioavailability of fat soluble micronutrients (fat soluble vitamins and carotenoids).
Detailed description
Healthy male volunteers were genotyped and submitted to 5 test-meals containing either no micronutrient of interest, 100 g tomato puree as a source of carotenoids, a lutein supplement, a vitamin E supplement or a vitamin D supplement. Blood samples were taken in the postprandial period (up to 8 hours after the test-meals intake) and the concentration of the studied micronutrients were measured in the chylomicrons. Multivariate analysis were then performed to identify single nucleotide polymorphisms that best explained the variance in the interindividual response (0 to 8 h area under the curve) in micronutrients.
Interventions
None listed
Sponsors
Institut National de la Santé Et de la Recherche Médicale, France
University of Marseille
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
Study design
Observational model
OTHER
Time perspective
OTHER
Eligibility
Sex/Gender
MALE
Age
18 Years to 60 Years
Healthy volunteers
Yes
Inclusion criteria
gender : male age : 18 to 60 years old BMI: \[18 to 29.9\] kg/m2 Fasting Cholesterol concentration : \< 2,5 g/l Fasting triglyceride concentration \< 2 g/l Fasting glycemia ≤ 1,1 g/l Fasting Hemoglobin \< 13 g/dl HIV as well as hepatitis C and B negative
Exclusion criteria
smokers regular consumption of vitamin supplement \> 140 g/week alcohol vegetarian diseases drugs assumed to affect the metabolism of lipids or fat soluble micronutrients (e.g. ezetimibe)
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| micronutrient concentration in plasma chylomicrons | 8 hours | The subjects were submitted to test-meals rich in micronutrients of interest and the ability of each subject to absorb the studied micronutrients was evaluated by measuring the postprandial chylomicron response (0 to 8 h area under the curve of the chylomicron micronutrient concentration) in each micronutrient. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| micronutrient concentration in adipose tissue | 8 hours | A first sample of adipose tissue was taken before the intake of the test-meal rich in fat soluble micronutrients. A second sample was taken 8 h after the test-meal intake. The aim was to assess whether the consumption of the studied fat soluble micronutrient modified the concentration of this micronutrient in adipose tissue. |
Countries
France
Outcome results
None listed