Sleep, Sleep Deprivation
Conditions
Brief summary
The aims of this study are examine the effects of sleep deprivation in muscle recovery after a maximum eccentric resistance exercise session performed on an isokinetic dynamometer (24 series of 10 repetitions). The sample will consist of 10 men, sedentary, clinically healthy, aged between 20 and 31 years old. Two experimental groups will be developed: EXE-SLEEP, in which subjects will perform the exercise protocol (\ 18:00-19:00) and will be subject to normal period of sleep for 3 nights; EXE-TOTAL, in which subjects will perform the same exercise protocol and will be sleep deprived for 60 hours, followed by one night of sleep rebound.
Detailed description
The blood parameters analyzed will be: * Creatine kinase and myoglobin: before, immediately after, 2, 4, 12, 24, 36, 48, and 60 hours after exercise protocol. * Total and free testosterone, IGF-1, and cortisol: before, immediately after, 2, 4, 12, 24, 36, 48, and 60 hours after exercise protocol. Moreover, from 19:00 of the second day to 19:00 of the third day it will be collected blood samples every 2 hours. * GH: during the second night, GH will be evaluated every 1 hour, from 23:00 to 07:00. * TNF-alfa, IL-6, IL-1 beta, IL-10, and IL-1ra: before, immediately after, and 2 hour after exercise, as well every 4 hours from 19:00 of the second day to 19:00 of the third day. It will be evaluated isometric strength every 12 hours, as well visual analog scale of pain.
Interventions
OTHERSleep deprivation
This group will perform 24 sets of 10 maximum repetitions of eccentric contractions for quadriceps and than will keep awake for 60 hours (2 nights), followed by 1 night of sleep, all inside the lab. During whole protocol, it will be collected several blood samples for evaluation of hormones, cytokines and muscle damage parameters. It will be also evaluated isometric strength and pain.
OTHERNormal sleep
This group will perform 24 sets of 10 maximum repetitions of eccentric contractions for quadriceps and and will be able to sleep the 3 nights. During whole protocol, it will be collected several blood samples for evaluation of hormones, cytokines and muscle damage parameters. It will be also evaluated isometric strength and pain.
Sponsors
Fundação de Amparo à Pesquisa do Estado de São Paulo
Federal University of São Paulo
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
MALE
Age
20 Years to 31 Years
Healthy volunteers
Yes
Inclusion criteria
* healthy subjects * sedentary * sleep duration between 6-8 hours peer night
Exclusion criteria
* smokers * use vitamin supplements, like C and E * drink two doses of alcohol/day or \> 4 doses in one occasion * use of anti-inflammatory drugs * antilipidemic drugs * anemia * muscle or joint diseases * cardiovascular diseases * diabetes * renal diseases * sleep disturbances * breathing disorders
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Change from baseline in isometric strength | Before exercise, immediatly after, 12, 24, 36, 48, and 60 hours after exercise induced-muscle damage |
Secondary
| Measure | Time frame |
|---|---|
| Area Under Curve for serum total and free testosterone, IGF-1, and cortisol | Blood samples will be collected every 2 hours, for 24 hours. |
Other
| Measure | Time frame |
|---|---|
| Area Under Curve for serum pro and anti-inflammatory cytokines | Blood samples will be collected every 4 hours, for 24 hours. |
Countries
Brazil
Outcome results
None listed