Aging
Conditions
Keywords
Green tea, aging, oxidation, anthropometrics.
Brief summary
The purpose of this study is to evaluate the beneficial effects of a long term (12 weeks) green tea drinking on plasma, erythrocytes and saliva oxidative stress biomarkers and antioxidant capacity, as well as on cardiometabolic risk factors: anthropometric, inflammation and metabolic parameters in a healthy aging men and women.
Detailed description
A prospective, crossover trial in which every individual served its own control. All subjects undergo a four weeks washout period, during which they briefed to avoid tea drinking and antioxidant supplements until the end of the study. At the end of the washout period, blood and saliva samples is collected after 12 hours of night fast. Subsequently, all subjects receive the maltodextrin containing placebo tea bags. The subjects instructed to brew the placebo sachets for five minute in 240 mL boiling water without stirring and to drink 4 cups per day for 12 weeks period (placebo period). After the placebo period, fasting samples collected once more and the subjects receive the green tea bags. Preparation and drinking instructions are the same as the placebo drink. At the end of the 12 weeks, green tea drinking, fasting blood and saliva samples drawn for the third time. During the study, every 4 weeks the subjects get additional placebo or green tea bags. Assessment of nutritional consumption of macronutrients and antioxidants using a food diary, physical activity lifestyle as well as weight, body fat and circumference measurements is made at the beginning, middle and end of the study.
Interventions
DIETARY_SUPPLEMENTFour green tea bags per day for 12 weeks
Four green tea bags per day for 12 weeks
OTHER4 daily placebo tea bags for 12 weeks
4 daily placebo tea bags for 12 weeks
Sponsors
Rambam Health Care Campus
Study design
Allocation
NON_RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
TREATMENT
Masking
SINGLE (Subject)
Eligibility
Sex/Gender
ALL
Age
60 Years to 76 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy men and women * Age: 60-76 Years * Able to understand the meaning of Informed Consent procedure and willing to consent
Exclusion criteria
* Any active disease state * Unstable chronic disease (diabetes, vascular, renal)
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Saliva antioxidant capacity | 24 weeks | Total antioxidant capacity, millimolar/mg |
| Plasma protein carbonyls | 24 weeks | Protein carbonyls, nmol/mg |
| Plasma thiobarbituric acid reactive substance | 24 weeks | Thiobarbituric acid reactive substance , nmol/mL |
| Plasma lipid peroxides | 24 weeks | Lipid peroxides, nmol/mL |
| Erythrocytes catalase | 24 weeks | Catalase activity, milliunits/mg |
| Erythrocytes resistance to oxidative hemolysis | 24 weeks | hemolysis test, percentage |
| Saliva enzymatic antioxidants | 24 weeks | Oral peroxidase activity, milliunits/mg |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Total body fat percentage | 24 weeks | Body fat, % |
| Body circumference | 24 weeks | Waist and hip circumference, cm |
| Serum inflammatory status | 24 weeks | Serum C-reactive protein mg/dL |
| Saliva inflammatory status Saliva matrix metalloproteinase 8 (MMP8) | 24 weeks | Saliva matrix metalloproteinase 8, µg/L |
| Saliva inflammatory status - saliva interleukin 8 (IL8) | 24 weeks | Saliva interleukin 8, ng/L |
| Total body weight | 24 weeks | Body weight, kg |
Countries
Israel
Outcome results
None listed