Body Composition, Beneficial, Insulin Resistance, Menopause
Conditions
Brief summary
The purpose of this study is to determine if physical exercise reduces the adverse changes in adipose tissue metabolism and insulin sensitivity that occurs in women during the menopausal transition.
Interventions
BEHAVIORALSpinning
After the baseline studies, the women will perform Spinning three times a week. Two of the sessions are performed on our location by trained instructors and the third training is self-training in a local fitness center where we provide free membership. All trainings are heart rate monitored.
Sponsors
Rigshospitalet, Denmark
Glostrup University Hospital, Copenhagen
University of Copenhagen
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
FEMALE
Age
45 Years to 57 Years
Healthy volunteers
Yes
Inclusion criteria
* Premenopausal OR Postmenopausal (45-57 years) * No chronical Diseases * BMI \> 18,5 and \< 30 * Sedentary (no regular physical activity the last 2 years) * Either regular bleeding (premenopausal) OR no menstruation for at least 2 years (postmenopausal) * Plasma measurements: Premenopausal: estradiol \> 0.20nmol/l, Follicle Stimulating Hormone \< 22 IU/l and progesterone \> 2.5 nmol/l Postmenopausal: estradiol \< 0.20nmol/l, Follicle Stimulating Hormone \> 22 IU/l and progesterone from 0.3-2.5 nmol/l
Exclusion criteria
* Smoking * Excessive alcohol intake * Regular medication including hormone replacement treatment
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Adipose tissue distribution | Baseline and 3 months | Accessed by Magnetic Resonance Imaging (MRI). |
| Insulin stimulated uptake of glucose in adipose tissue | Baseline and 3 months | Measured by Positron Emission Tomography-Computed tomography (PET/CT) |
| Hyperinsulinemic euglycemic clamp | Baseline and 3 months | Glucose metabolized (mg/kg/min) |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Metabolic syndrome | Baseline and 3 months | As measured by high density lipoprotein, triglycerides, waist circumference, blood pressure, mean arterial pressure, fasting glucose and composite metabolic syndrome scores |
| Health related quality of life | Baseline and 3 months | Measured using questionaries, semi-structured interviews and observations |
| Anthropometry | Baseline and 3 months | Measured using dual x-ray absorptiometry, height, weight, waist and hip circumference, sagittal abdominal height |
| Skeletal muscle biopsy | Baseline and 3 months | Biochemical, proteomics, metabolomics, genomics and morphological analyses |
| Resting metabolic rate | Baseline and 3 months | Will be measured by indirect calorimetry |
| Physical activity level and duration of sleep | Baseline and 3 months | Daily activity and duration of sleep will be recorded during one week using Actibelt® and self reporting |
| Microdialysis | Baseline and 3 months | Adipose tissue microdialysis |
| Femoral subcutaneous adipose tissue biopsy | Baseline and 3 months | Biochemical, proteomics, metabolomics, genomics and morphological analyses |
| Abdominal subcutaneous adipose tissue biopsy | Baseline and 3 months | Biochemical, proteomics, metabolomics, genomics and morphological analyses |
| Central insulin sensitivity | Baseline and 3 months | Fasting plasma insulin, homeostasis model assessment insulin resistance and measures of central insulin sensitivity derived from an oral glucose tolerance test |
| Maximal oxygen uptake (ml/O2/kg/min) | Baseline and 3 months | Measured using indirect calorimetry and an incremental bicycle protocol |
Other
| Measure | Time frame | Description |
|---|---|---|
| Dietary registration | Baseline and 3 months | Daily food intake for three week days and one week-end day will be reported by the subjects |
Countries
Denmark
Outcome results
None listed