The Influence of Different Concentrations of Glucose in Culture Media on In Vitro Fertilization (IVF) Outcome of Sibling Oocytes

The Influence of Different Concentrations of Glucose in Culture Media on In Vitro Fertilization Outcome of Sibling Oocytes

Status

UNKNOWN

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT02066584

Enrollment

600

Registered

2014-02-19

Start date

2014-04-30

Completion date

2016-04-30

Last updated

2014-02-19

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Infertility

Keywords

sibling oocytes, glucose, culture media

Brief summary

The importance of glucose in culture media on fertilization and embryo development is still controversial. Although it has been reported that glucose is essential for fertilization process and biosynthesis of membrane lipid and nucleic acids, comparatively high levels of glucose in culture media may cause developmental arrest of cleavage stage embryos. The aim of this retrospective study is to examine whether exposure of sibling oocytes to different concentrations of glucose in culture media has any further effect on IVF outcome.

Detailed description

This retrospective study will include data from either standard IVF or Intracytoplasmic Sperm Injection (ICSI) cycles, in which sibling oocytes were equally divided and incubated separately in one of the following pairs of culture media: 1. Universal IVF medium vs. ISM1 medium ( Origio, Medi-Cult) 2. P1 medium vs. ECM medium (Irvine). Fertilization rate,as well as embryo cleavage rate and morphology will be evaluated.

Interventions

OTHERSibling oocyte incubation

Study design

Observational model

COHORT

Time perspective

RETROSPECTIVE

Eligibility

Sex/Gender

FEMALE

Age

18 Years to 42 Years

Healthy volunteers

Inclusion criteria

* Sibling oocytes from cycles where at least 4 oocytes were retrieved

Exclusion criteria

* Less than 4 oocytes retrieved * Partner has undergone testicular sperm extraction (TESE)

Design outcomes

Primary

Measure	Time frame	Description
Fertilization Rate	2 years	Fertilization rates will be retrospectively compared according to culture media in which sibling oocytes were incubated

Secondary

Measure	Time frame	Description
Embryo Quality	2 years	Embryo quality and developmental rate will be retrospectively compared according to culture media in which sibling oocytes were incubated

Countries

Israel

Contacts

Primary ContactMedeia Michaeli, PhD

medeia_michaeli@yahoo.com972-50-6246885

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026