Alteration of Mitochondrial Membrane, Autoimmune Thyroid Disease, Subclinical Hypothyroidism
Conditions
Keywords
Mitochondria Function, TPO-antibodies, Subclinical Thyroid Disease, Thyroid Disease In Pregnancy, Children´s Development
Brief summary
Previously, studies have shown that children of women with thyroid autoantibodies experience more birth complications and poorer health in their first days. Studies have also shown later signs of cognitive developmental challenges (risk of attention deficit/hyperactivity problems) among children of mothers with autoimmune thyroid disease and/or subclinical hypothyroidism. In Denmark there is no formalized screening or treatment of subclinical thyroid disease - with or without Thyroid Peroxidase Antibodies (TPO-antibodies) - among pregnant women. The hypothesis of this study is that the offspring of women with subclinical thyroid disease have a mitochondria-dysfunction which leads to more complications during birth, poorer health and well-being in the early childhood. The investigators will test this by recruiting mothers by a blood sample in the third trimester of pregnancy, screen the cord blood at birth and later on test the children with Bayley test two times in the early childhood.
Detailed description
Prior to a planned caesarean section, maternal blood samples are drawn and at the cesarean, cord blood samples are drawn, when the cord is clamped and cut. Thyrotropin, free T3, free T4, anti-TPO and lipids are measured on maternal as well as cord samples. Flow cytometry is performed to measure mitochondrial function. At age 6 months and 15 months the child´s development is evaluated by the Bayley-III test.
Interventions
None listed
Sponsors
University of Southern Denmark
Region Zealand
Naestved Hospital
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 50 Years
Healthy volunteers
No
Inclusion criteria
* Singleton pregnancy, clinically healthy
Exclusion criteria
* Twin-pregnancy, metabolic disorder, medication or other diseases with a potential adverse impact on the pregnancy and fetus
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Mitochondrial function | Delivery | Maternal and cord blood. Analyses will be run by flow cytometry and qPCR |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Well-being | Age 0-15 months | Number of children in each group that have been admitted to the hospital due to icterus or metabolic disease |
| Weight (kg) | Age 0-15 months | Differences between the two groups |
| Length (cm) | Age 0-15 months | Differences between the two groups |
| Head circumference (cm) | Age 0-15 months | Differences between the two groups |
| Perinatal complications | At birth | Number of children in each group with abnormal apgar score, cord pH, need of CPAP, resuscitation, low blood sugar, cramps, death |
| Cognitive development | Age 6 and 15 months | Differences between the two groups, evaluated by Bayley test |
| Language | Age 6 and 15 months | Differences between the two groups, evaluated by Bayley test |
| Birth complications | Birth | Number of birth complications in the two groups in terms of postpartum hemorrhage \>=500 ml |
| Social/emotional behavior | Age 12 months | Differences between the two groups, evaluated by ASQ:SE |
| Motor development | Age 6 and15 months | Differences between the two groups, evaluated by Bayley test |
Countries
Denmark
Outcome results
None listed