Couple Sterility
Conditions
Keywords
Myoinositol, ART, EMBRYO QUALITY
Brief summary
In the present trial, the investigators aim to evaluate whether semen myo-inositol (MI) treatment is able to improve IVF outcomes. In particular, retrieved oocytes will be randomly divided in two groups, one group will be inseminated with MI treated semen while the other will be inseminated with untreated semen.
Detailed description
Myo is synthesized from glucose-6-phosphate, it is a precursor of second messengers in the cell signaling pathways and it is involved in the regulation of intracellular calcium. Literature data have demonstrated the MI play a crucial role in reproduction, indeed, oocyte and spematozoa are nursed in a medium congaing hig MI concentration. Furthermore, it has been demonstrated that, in oligoasthenoteratozoospermic patients, in vitro incubation with 2 mg/ml of myo increase significantly the number of spermatozoa with high mitochondrial membrane potential (MMP). Moreover, it has been found that the in vitro fertilization rate is correlated with the percentage of high MMP spermatozoa.
Interventions
DIETARY_SUPPLEMENTMyo-inositol
OTHERuntreated
Sponsors
AGUNCO Obstetrics and Gynecology Centre
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
OTHER
Masking
SINGLE (Outcomes Assessor)
Eligibility
Sex/Gender
MALE
Age
30 Years to 42 Years
Healthy volunteers
No
Inclusion criteria
* couple counselled for IVF
Exclusion criteria
* cryopreserved sample
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Fetilization rate | Day 1 |
Secondary
| Measure | Time frame |
|---|---|
| Embryo quality | Day 2 to Day 5 |
Countries
Italy
Outcome results
None listed