Observation of Microvessels and Invasion in Early Colorectal Lesions by NBI

Observation of Microvessels and Invasion in Early Colorectal Lesions by Narrow Band Imaging:Combination With Microvessel Count and MMP-7 Expression

Status

Completed

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT02047188

Enrollment

418

Registered

2014-01-28

Start date

2010-01-31

Completion date

2013-12-31

Last updated

2014-01-28

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Colorectal Neoplasms

Keywords

narrow band imaging,microvessels,matrix metalloproteinase-7

Brief summary

Combined with magnifying endoscopy,narrow-band imaging (NBI) contrasts microvascular architecture on lesion surface.The histology of early colorectal lesions could be predicted under NBI view.However,its capability for estimating invasion depth remains to be verified.The study is based on the hypothesis:NBI can predict histology and invasion depth,combined with the verification of microvessel count and MMP-7 expression.

Detailed description

The microvessel count (MVC) is a classical method of assessing histologic grade.To verify whether NBI could predict histology through microvascular architecture,we measured lesion microvessels by immunohistochemistry.On the other hand,we applied immunohistochemistry of matrix metalloproteinase-7 (MMP-7) to validate the capability of NBI for estimating invasion depth.MMP-7 is directly involved in the processes of growth, invasion, and metastasis of colorectal cancer.Our target was to clarify the diagnostic accuracy of magnifying NBI in early colorectal lesions,combined with the verification of microvessel count and MMP-7 expression.

Interventions

None listed

Study design

Observational model

CASE_ONLY

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

ALL

Age

18 Years to 90 Years

Healthy volunteers

Inclusion criteria

* patients of early colorectal lesions with capillary pattern(CP) type II&III,initially detected by white-light view and then examined by NBI with magnifying endoscopy.

Exclusion criteria

* lesions with CP type I; * CP type III lesions with an obvious appearance of advanced cancer; * lesions that had underwent biopsy; * patients with inflammatory bowel disease(IBD) or familial adenomatous polyposis(FAP) * patients with previous colorectal surgery; * having conditions associated with cardiac,hepatic,renal,and coagulopathy diseases.

Design outcomes

Primary

Measure	Time frame	Description
NBI prediction for lesion histology and invasion	in two weeks after lesion treatment	We will receive the histologic findings in two weeks after lesion treatment.Then we can compare NBI prediction with the final histologic type.

Secondary

Measure	Time frame	Description
immunohistochemical outcome of microvessel count and MMP-7	in three days after immunohistochemistry	We will receive the immunohistochemical outcome in three days after immunohistochemistry.Then we can compare NBI prediction with immunohistochemical outcome

Outcome results