Type 1 and 2 Diabetes
Conditions
Brief summary
* Our research hypothesis is to show that a certain number of genetic polymorphisms of the proteins involved in glucose, lipid and adipocyte metabolism are factors that favour the development of steatosis in patients with Type 2 diabetes. * We also wish to evaluate more thoroughly lipid anomalies associated with the presence of steatosis, notably with regard to monocyte expression of LDL receptors. We hypothesize that hepatic steatosis is accompanied by activation of transcription factors involved in lipogenesis, notably SREBP factors. The activation of these factors could cause an increase in the expression of LDL receptors, leading to increased LDL catabolism. * Chronological description of the study During an outpatient consultation at the endocrinology department, diabetic patients, programmed to undergo an examination to assess their diabetes will be invited to participate in the study. Once written informed consent has been provided and clinical data has been recorded, patients with type 1 or type 2 diabetes will have standard biological examination, which is systematically done in such patients (Fasting glycemia, HBA1c, aspartate aminotransferase, alanine amino transferase, Gammaglutamyl-transferases, PAL, bilirubin, blood proteins, albuminemia, Total Cholesterol total, HDL cholesterol, triglycerides, Sedimentation Rate, C-reactive protein, fibrinogen). As well as the systematic biological tests, 3 additional tubes will be taken to screen for genetic polymorphism in 3 proteins (Microsomal Transfer Protein, Adiponectin receptor - 1, Apolipoprotein A - II). IN addition, magnetic resonance imaging and magnetic resonance spectroscopy will be done to look for the presence of liver steatosis and to measure carotid intima-media thickness.
Interventions
OTHERprise de sang
OTHERmagnetic resonance imaging and magnetic resonance spectroscopy
Sponsors
Centre Hospitalier Universitaire Dijon
Study design
Observational model
OTHER
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
Inclusion criteria Type-2 diabetics: * Type 2 diabetes * HbA1C\>6.5% * 27\<BMI\<55 Inclusion criteria Type-1 diabetics: * Type 1 diabetes * BMI\<55 Diagnosis of type-1 diabetes based on the clinical history of the patient and/or the presence of anti-glutamate decarboxylase auto antibodies and/or a plasma level of C peptide below 0,5 ng/l. Inclusion criteria healthy volunteers: * Non diabetic * Alcohol consumption \< 2 glasses per day * Without hyperglycemic treatment (corticoids, ...) * Without liver disease (cirrhosis, hepatitis, ...)
Exclusion criteria
* Pacemaker * Daily alcohol consumption above 4 glasses per day * Patients treated with Glitazones during the 3 months preceding inclusion * Presence of implants * Claustrophobia * Patient \< 18 years * Patient under guardianship or not intellectually independent * Pregnancy
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Determination of the existence of liver steatosis measured by magnetic resonance spectroscopy spectrometry | At inclusion |
Secondary
| Measure | Time frame |
|---|---|
| Measurement of monocyte expression of LDL receptors | At inclusion |
Countries
France
Outcome results
None listed