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Bortezomib and Filgrastim to Promote Stem Cell Mobilization in Patients With Non-Hodgkin Lymphoma or Multiple Myeloma

A Pilot Study of Peripheral Blood Hematopoietic Stem Cell Mobilization With the Combination of Bortezomib and G-CSF in Multiple Myeloma and Non-Hodgkin's Lymphoma Patients

Status
Completed
Phases
NA
Study type
Interventional
Source
ClinicalTrials.gov
Registry ID
NCT02037256
Enrollment
23
Registered
2014-01-15
Start date
2011-07-31
Completion date
2015-01-21
Last updated
2021-05-10

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Adult Grade III Lymphomatoid Granulomatosis, B-cell Chronic Lymphocytic Leukemia, Contiguous Stage II Adult Burkitt Lymphoma, Contiguous Stage II Adult Diffuse Large Cell Lymphoma, Contiguous Stage II Adult Diffuse Mixed Cell Lymphoma, Contiguous Stage II Adult Diffuse Small Cleaved Cell Lymphoma, Contiguous Stage II Adult Immunoblastic Large Cell Lymphoma, Contiguous Stage II Adult Lymphoblastic Lymphoma, Contiguous Stage II Grade 1 Follicular Lymphoma, Contiguous Stage II Grade 2 Follicular Lymphoma, Contiguous Stage II Grade 3 Follicular Lymphoma, Contiguous Stage II Mantle Cell Lymphoma, Contiguous Stage II Marginal Zone Lymphoma, Contiguous Stage II Small Lymphocytic Lymphoma, Cutaneous B-cell Non-Hodgkin Lymphoma, Extranodal Marginal Zone B-cell Lymphoma of Mucosa-associated Lymphoid Tissue, Intraocular Lymphoma, Nodal Marginal Zone B-cell Lymphoma, Noncontiguous Stage II Adult Burkitt Lymphoma, Noncontiguous Stage II Adult Diffuse Large Cell Lymphoma, Noncontiguous Stage II Adult Diffuse Mixed Cell Lymphoma, Noncontiguous Stage II Adult Diffuse Small Cleaved Cell Lymphoma, Noncontiguous Stage II Adult Immunoblastic Large Cell Lymphoma, Noncontiguous Stage II Adult Lymphoblastic Lymphoma, Noncontiguous Stage II Grade 1 Follicular Lymphoma, Noncontiguous Stage II Grade 2 Follicular Lymphoma, Noncontiguous Stage II Grade 3 Follicular Lymphoma, Noncontiguous Stage II Mantle Cell Lymphoma, Noncontiguous Stage II Marginal Zone Lymphoma, Noncontiguous Stage II Small Lymphocytic Lymphoma, Progressive Hairy Cell Leukemia, Initial Treatment, Recurrent Adult Burkitt Lymphoma, Recurrent Adult Diffuse Large Cell Lymphoma, Recurrent Adult Diffuse Mixed Cell Lymphoma, Recurrent Adult Diffuse Small Cleaved Cell Lymphoma, Recurrent Adult Grade III Lymphomatoid Granulomatosis, Recurrent Adult Immunoblastic Large Cell Lymphoma, Recurrent Adult Lymphoblastic Lymphoma, Recurrent Grade 1 Follicular Lymphoma, Recurrent Grade 2 Follicular Lymphoma, Recurrent Grade 3 Follicular Lymphoma, Recurrent Mantle Cell Lymphoma, Recurrent Marginal Zone Lymphoma, Recurrent Small Lymphocytic Lymphoma, Refractory Hairy Cell Leukemia, Refractory Multiple Myeloma, Small Intestine Lymphoma, Splenic Marginal Zone Lymphoma, Stage I Adult Burkitt Lymphoma, Stage I Adult Diffuse Large Cell Lymphoma, Stage I Adult Diffuse Mixed Cell Lymphoma, Stage I Adult Diffuse Small Cleaved Cell Lymphoma, Stage I Adult Immunoblastic Large Cell Lymphoma, Stage I Adult Lymphoblastic Lymphoma, Stage I Grade 1 Follicular Lymphoma, Stage I Grade 2 Follicular Lymphoma, Stage I Grade 3 Follicular Lymphoma, Stage III Adult Burkitt Lymphoma, Stage III Adult Diffuse Large Cell Lymphoma, Stage III Adult Diffuse Mixed Cell Lymphoma, Stage III Adult Diffuse Small Cleaved Cell Lymphoma, Stage III Adult Immunoblastic Large Cell Lymphoma, Stage III Adult Lymphoblastic Lymphoma, Stage III Grade 1 Follicular Lymphoma, Stage III Grade 2 Follicular Lymphoma, Stage III Grade 3 Follicular Lymphoma, Stage III Mantle Cell Lymphoma, Stage III Marginal Zone Lymphoma, Stage III Multiple Myeloma, Stage III Small Lymphocytic Lymphoma, Stage II Multiple Myeloma, Stage I Mantle Cell Lymphoma, Stage I Marginal Zone Lymphoma, Stage I Multiple Myeloma, Stage I Small Lymphocytic Lymphoma, Stage IV Adult Burkitt Lymphoma, Stage IV Adult Diffuse Large Cell Lymphoma, Stage IV Adult Diffuse Mixed Cell Lymphoma, Stage IV Adult Diffuse Small Cleaved Cell Lymphoma, Stage IV Adult Immunoblastic Large Cell Lymphoma, Stage IV Adult Lymphoblastic Lymphoma, Stage IV Grade 1 Follicular Lymphoma, Stage IV Grade 2 Follicular Lymphoma, Stage IV Grade 3 Follicular Lymphoma, Stage IV Mantle Cell Lymphoma, Stage IV Marginal Zone Lymphoma, Stage IV Small Lymphocytic Lymphoma, Untreated Hairy Cell Leukemia, Waldenström Macroglobulinemia

Brief summary

This clinical trial studies peripheral blood hemapoietic stem cell mobilization with the combination of bortezomib and G-CSF (filgrastim) in multiple myeloma and non-Hodgkin lymphoma patients.

Detailed description

PRIMARY OBJECTIVES: I. To estimate if addition of Bortezomib to the mobilization protocol will result with an increase in the levels of circulating peripheral blood stem cells (PBSCs) by at least 2-fold in blood and in the apheresis collections in up to 4-days collection protocol. II. To assess whether time to neutrophil engraftment is 12 days or less, the historical value. SECONDARY OBJECTIVES: I. To test for co-mobilization of lymphoma or myeloma cells by bortezomib and G-CSF using real time polymerase chain reaction (PCR) for non-Hodgkin lymphoma (NHL) patients and by flow cytometry (cluster of differentiation \[CD\]38+/CD138+ cell) for multiple myeloma (MM) patients. II. To determine the effect of Bortezomib on the extent of mobilization of dendritic cells subsets, plasmacytoid dendritic cell (pDC)1 and pDC2 and DC1/DC2 ratio by flow cytometry.

Interventions

DRUGbortezomib

Given IV

BIOLOGICALfilgrastim

Given SC

PROCEDUREautologous hematopoietic stem cell transplantation

Undergo autologous hematopoietic stem cell transplantation

Sponsors

National Cancer Institute (NCI)
CollaboratorNIH
Barbara Ann Karmanos Cancer Institute
Lead SponsorOTHER

Study design

Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
NONE

Eligibility

Sex/Gender
ALL
Age
18 Years to 70 Years
Healthy volunteers
No

Inclusion criteria

* Voluntary written informed consent form before performance of any study-related procedure not part of normal medical care, with the understanding that consent may be withdrawn by the subject at any time without prejudice to future medical care * Diagnosis of B-type NHL or with multiple myeloma and eligible for autologous transplantation * No more than 3 prior regimens of chemotherapy (Rituximab is not considered chemotherapy) and 4 weeks out of Bortezomib treatment for MM * Karnofsky performance status of \> 50% * The patient has recovered from all acute toxic effects of prior chemotherapy * White blood cell (WBC) \> 3.0 x 10\^9/L * Absolute neutrophil count \> 1.5 x 10\^9/L * Platelet count \> 100 x 10\^9/L * Serum creatinine =\< 2.2 * Serum glutamic oxaloacetic transaminase (SGOT) and serum glutamic pyruvate transaminase (SGPT) less than two times the upper limit of normal (ULN) * Total bilirubin less than two times the ULN * Left ventricle ejection fraction \> 50% (by normal echocardiogram \[ECHO\] or multi gated acquisition scan \[MUGA\] scan) * Diffusing capacity of the lung for carbon monoxide (DLCO) \> 50% * Forced vital capacity \> 50% of predicted * Negative for human immunodeficiency virus (HIV) * Female subject is either post-menopausal for at least 1 year before the screening visit, is surgically sterilized or if they are of childbearing potential , agree to use 2 effective methods of birth control (i.e., a hormonal contraceptive, intra-uterine device, diaphragm with spermicide, condom with spermicide, or abstinence) from the time of signing the informed consent form through 30 days after the last dose of Bortezomib, or agree to completely abstain from heterosexual intercourse; women of child bearing potential agree to use an approved form of contraception; male subject, even if surgically sterilized (i.e., status post-vasectomy) must agree to 1 of the following: practice effective barrier contraception during the entire study treatment period and through a minimum of 30 days after the last dose of study drug, or completely abstain from heterosexual intercourse for the duration of the study

Exclusion criteria

* Patient has a platelet count of \< 100x 10\^9/L within 14 days before enrollment * Patient has an absolute neutrophil count of ANC \<1.5 x 10\^9/L within 14 days before enrollment * Patient has creatinine of \> 2.2 MG/DL within 14 days before enrollment * Patient has \> 1.5 x ULN total bilirubin * Patient has \>= grade 2 peripheral neuropathy within 14 days before enrollment * Myocardial infarction within 6 months prior to enrollment or has New York Heart Association (NYHA) class III or IV heart failure, uncontrolled angina, severe uncontrolled ventricular arrhythmias, or electrocardiographic evidence of acute ischemia or active conduction system abnormalities; prior to study entry, any electrocardiogram (ECG) abnormality at screening has to be documented by the investigator as not medically relevant * Patient has hypersensitivity to Bortezomib, boron or mannitol * Female subject is pregnant or breast-feeding; confirmation that the subject is not pregnant must be established by a negative serum beta-human chorionic gonadotropin (b-hCG) pregnancy test result obtained during screening; pregnancy testing is not required for post-menopausal or surgically sterilized women * Participation in clinical trials with other investigational drugs not included in this trial, within 14 days before enrollment and throughout the duration of this trial * Serious medical or psychiatric illness likely to interfere with participation in this clinical study * A co-morbid condition which, in the view of the investigators, renders the patient at high risk for this study * An acute medical condition resulting from prior chemotherapy * Brain metastases or carcinomatous meningitis * Acute infection * Fever (temp \> 38 degrees Celsius \[C\]/100.4 degrees Fahrenheit \[F\]) * Patients of child-bearing potential unwilling to implement adequate birth control * Patients who have deterioration of their clinical status or laboratory parameters between the time of enrollment and transplant (such that they no longer meet entry criteria) may be removed from study at the discretion of the treating physician or principal investigator * Diagnosed or treated for another malignancy within 3 years of enrollment, with the exception of complete resection of basal cell carcinoma or squamous cell carcinoma of the skin, an in situ malignancy, or low-risk prostate cancer after curative therapy * Radiation therapy within 3 weeks before randomization; enrollment of subjects who require concurrent radiotherapy (which must be localized in its field size) should be deferred until the radiotherapy is completed and 3 weeks have elapsed since the last date of therapy

Design outcomes

Primary

MeasureTime frameDescription
>= 2 Fold Increase in Circulating PBSC'sUp to 6 monthsParticipants with \>= 2 fold increase in circulating PBSC's in blood and in apheresis collections in up to 4-days collection

Secondary

MeasureTime frameDescription
Time to Neutrophil EngraftmentUp to 6 monthsEstimated Median Time to Neutrophil Engraftment, ANC 500

Countries

United States

Participant flow

Participants by arm

ArmCount
Arm A
Pts. receive filgrastim SC on days 1-9 & begin apheresis on day 5. Pts. undergo apheresis for up to 2 days, & receive bortezomib intravenously (IV) over 3-5 seconds after target collection is obtained with filgrastim alone or on the 1st day of collection with the Bortezomib plus filgrastim mobilization, prior to receiving the administration of filgrastim. 2nd apheresis will continue until target stem cell dose is reached or for maximum 4 days. Pts. undergo autologous hematopoietic stem cell transplantation after receiving high dose chemotherapy & peripheral blood stem cell (PBSC) infusion following standard of care procedures. bortezomib: Given IV filgrastim: Given SC autologous hematopoietic stem cell transplantation: Under
3
Arm B
Pts. receive filgrastim SC on days 1-8 & receive bortezomib IV over 3-5 seconds on days 4 & day 7, before administration of filgrastim. Pts. undergo apheresis on days 5-8
17
Total20

Withdrawals & dropouts

PeriodReasonFG000FG001
Overall StudyProtocol Violation01
Overall StudyWithdrawn prior to Velcade02

Baseline characteristics

CharacteristicArm BTotalArm A
Age, Categorical
g
<=18 years
0 Participants0 Participants0 Participants
Age, Categorical
g
>=65 years
4 Participants5 Participants1 Participants
Age, Categorical
g
Between 18 and 65 years
13 Participants15 Participants2 Participants
Age, Continuous56 years58 years62 years
Race (NIH/OMB)
American Indian or Alaska Native
0 Participants0 Participants0 Participants
Race (NIH/OMB)
Asian
0 Participants0 Participants0 Participants
Race (NIH/OMB)
Black or African American
3 Participants4 Participants1 Participants
Race (NIH/OMB)
More than one race
0 Participants0 Participants0 Participants
Race (NIH/OMB)
Native Hawaiian or Other Pacific Islander
0 Participants0 Participants0 Participants
Race (NIH/OMB)
Unknown or Not Reported
0 Participants0 Participants0 Participants
Race (NIH/OMB)
White
14 Participants16 Participants2 Participants
Region of Enrollment
United States
17 Participants20 Participants3 Participants
Sex: Female, Male
Female
9 Participants11 Participants2 Participants
Sex: Female, Male
Male
8 Participants9 Participants1 Participants

Adverse events

Event typeEG000
affected / at risk
EG001
affected / at risk
deaths
Total, all-cause mortality
0 / 60 / 17
other
Total, other adverse events
6 / 617 / 17
serious
Total, serious adverse events
0 / 60 / 17

Outcome results

Primary

>= 2 Fold Increase in Circulating PBSC's

Participants with \>= 2 fold increase in circulating PBSC's in blood and in apheresis collections in up to 4-days collection

Time frame: Up to 6 months

Population: Evaluable patients

ArmMeasureGroupValue (COUNT_OF_PARTICIPANTS)
Group A: Bortezomib 1.3 mg/m2>= 2 Fold Increase in Circulating PBSC'sDoubling0 Participants
Group A: Bortezomib 1.3 mg/m2>= 2 Fold Increase in Circulating PBSC'sLess than doubling3 Participants
Group B Bortezomib 1/3 mg/m2>= 2 Fold Increase in Circulating PBSC'sDoubling4 Participants
Group B Bortezomib 1/3 mg/m2>= 2 Fold Increase in Circulating PBSC'sLess than doubling13 Participants
Secondary

Time to Neutrophil Engraftment

Estimated Median Time to Neutrophil Engraftment, ANC 500

Time frame: Up to 6 months

Population: Evaluable patients

ArmMeasureValue (MEDIAN)
Group A: Bortezomib 1.3 mg/m2Time to Neutrophil Engraftment12 days to ANC 500
Group B Bortezomib 1/3 mg/m2Time to Neutrophil Engraftment13 days to ANC 500
Comparison: Median time to engraftment95% CI: [0.07, 0.5]

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026