Air Pollution
Conditions
Keywords
air pollution, food, nitrites, nitrates, breath tests/methods
Brief summary
The study hypothesis is that drinking different juices will affect the body's responses to air pollution. Subjects will be exposed to air pollution during a 2-hour car ride on the NJ (New Jersey) Turnpike. Each subject will be asked to do this twice. Before one car ride, the subject will be asked to drink orange juice. Before the other car ride, the subject will be asked to drink beet juice. Samples of blood and exhaled breath will be collected before, immediately after, and 24 hours after each car ride. Levels of nitrites/nitrates will be measured in the blood and breath.
Detailed description
Subjects will participate in two exposure sessions. The air pollution exposure consists of a 2-hour car ride along the NJ Turnpike. Samples will be collected at 3 time points for each exposure session: pre-exposure, immediately post-exposure, and 24 hours post-exposure. Blood samples will be collected immediately before and after a 5-minute occlusion at each time point. The subject will also be asked to provide samples of exhaled breath and complete symptom and stress questionnaires at each time point. Blood samples will be analyzed for serum nitrite. Exhaled breath samples will be analyzed for nitrates and nitrites.
Interventions
DIETARY_SUPPLEMENTBeet juice
2 cups of beet juice will be given before the car ride
DIETARY_SUPPLEMENTOrange juice
2 cups of orange juice will be given before the car ride
Sponsors
Rutgers, The State University of New Jersey
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy * Must live within 20 miles of Piscataway, NJ
Exclusion criteria
* • Are a current smoker * Are pregnant or breastfeeding * Are HIV-positive * Have an active medical condition such as high blood pressure, asthma, diabetes or other serious disease * Have a heart condition such as atrial flutter, atrial fibrillation or a pacemaker * Are unable to ride comfortably in a car for 2 hours * Travel to the EOHSI (Environmental and Occupational Health Sciences Institute) Clinic Center on major highways for more than 3 miles (the Garden State Parkway, the New Jersey Turnpike, Routes 287, 1, 18, or 9).
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in plasma nitrite concentration | Prior to the car ride, immediately after the 2-hour car ride, and 24 hours later | Change in plasma nitrite concentration pre- and post-occlusion |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Change in nitrite/nitrate concentration in exhaled breath | Prior to the car ride, immediately after the 2 hour car ride and 24 hours later | Change in nitrite/nitrate concentration in exhaled breath |
Countries
United States
Outcome results
None listed