Male Infertility
Conditions
Keywords
Azoospermia, Spermatogonial Arrest, Maturation Arrest.
Brief summary
In this study, we performed autologous BMDMSC transplantation to the testis of Azoospermic patients in a trial to enhance and activate the spermatogonial stem cells of the testis and aiming to produce motile sperm.
Detailed description
Bone marrow aspirated and stem cells were isolated, cultured and characterized using flow cytometer. Stem cells prepared according to GMP regulations then injected into rete testis using special syringe. Patients follow up will carried out for a year , three month interval. FSH, testosterone, testicular size as well as sexual potency will assessed every three months.
Interventions
BIOLOGICALStem Cells
60 ml of bone marrow will aspirated and used for stem cells isolation. Then stem cells will cultured using autologous serum, characterized and prepared using GMP rules and finally injected into rete testis.
Sponsors
Cairo University
Al-Azhar University
Study design
Allocation
RANDOMIZED
Intervention model
SINGLE_GROUP
Primary purpose
TREATMENT
Masking
SINGLE (Subject)
Eligibility
Sex/Gender
MALE
Age
20 Years to 50 Years
Healthy volunteers
No
Inclusion criteria
* All Non obstructive Azoospermic Patients. * Azoospermic Patients due to Administration of Chemo/Radiotherapy.
Exclusion criteria
* Patients with Testicular Cancer. * Patients with Complete Deletion of Chromosome Y AZF (a,b,c) Regions.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| All Cases Improvement | One Year (12-Month) | Hormonal Assessment: Serum (Testosterone , FSH, LH and Prolactin). Testicular Size. Sexual Potency. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| All Cases Fertility Improvement | One Year (12-Month) | Hormonal Assessment: Serum (Testosterone , FSH, LH and Prolactin) Testicular size and Sexual Potency. |
Countries
Egypt
Outcome results
None listed