The Role of TLR-4 Polymorphisms in Children With Urinary Track Infections

The Role of TLR-4 Asp299Gly and Thr399Ile Polymorphisms in Children With Urinary Tack Infections

Status

UNKNOWN

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT02021305

Acronym

TLR-4

Enrollment

160

Registered

2013-12-27

Start date

2013-02-28

Completion date

2017-02-28

Last updated

2013-12-27

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Urinary Tract Infection

Keywords

Toll like Receptors, TLR 4, Urinary track infections in children, TLR 4 polymorphisms, Innate immunity, Defence mechanisms, Immune responses

Brief summary

Purpose So far anatomical abnormalities (mostly congenital) were, in the majority of the patients, associated with urinary track infections. In this study the researchers will try to investigate the role of TLRs as molecular interactions between bacterial virulence and host response. TLRs are important mediators in the development of the natural immunity against bacteria. They recognize microbial pathogen associated molecular patterns and alert the host's immune system to the presence of invading microbes

Detailed description

The aim of his observational case-control study is to : 1. investigate if the expression of TLR2 and TLR4 mediate in the development of the natural immunity of the uroepithileal cells against bacteria. 2. evaluate the effect of the TLR-gene Asp299Gly and Thr399Ile polymorphisms on the development and clinical severity of urinary tract infections (UTI) in children. 3. determine the association of the expression of TLR-2 and TLR-4 and TLR-4 polymorphisms with the formation of renal scar in children. 4. investigate the expression of TLR-2 and TLR-4 and TLR-4 polymorphisms in healthy children and elaborate the results to the possible carriers of these polymorphisms.

Interventions

None listed

Study design

Observational model

CASE_CONTROL

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

ALL

Age

6 Months to 14 Years

Healthy volunteers

Yes

Inclusion criteria

* For the patients * 6months -14 years of age * unrelated male and female children with one episode of acute urinary track infection (positive urine culture and clinical symptoms ) * with/without anatomic genitourinary anomalies . * children with upper UTI or children with lower UTI * Sign written informed consent Inclusion Criteria: For the controls * 6 months-14 years of age * age and gender matched with the patients * unrelated male and female children with no recorded history of urinary track infection and negative urine cultures . * Sign written informed consent

Exclusion criteria

* For the patients * Subjects who do not meet the criteria above * Severe or chronic illness * Symptoms of acute infection * Immune deficiency or immune suppressor therapy * Medication

Design outcomes

Primary

Measure	Time frame
frequency of TLR-4 299, 399 polymorphism and expression of TLR-2 and TLR-4 by monocytes in children with urinary track infection and healthy controls.	4 years

Countries

Greece

Contacts

Primary ContactEfimia Papadopoulou-Alataki, MD, PhD

efiala@otenet.gr+302313323901

Backup ContactPanagiota Karananou, MD

yiotar@hotmail.com+302313323901

Outcome results