Healthy Adults
Conditions
Keywords
lysine, bioavailability, amino acids, corn
Brief summary
Our objective is to determine the metabolic availability of Lysine in white maize using the indicator amino acid oxidation (IAAO) technique in adult men.
Detailed description
Our objective is to determine the metabolic availability of Lysine in white maize. 7 young, healthy, male adults will receive graded levels (29, 37,46 and 54%) of lysine requirement of 35 mg/kg/d as a crystalline AA mixture and a porridge of white cornmeal protein respectively.
Interventions
DIETARY_SUPPLEMENTCrystalline Lysine Amino Acid Mix
Crystalline Lysine from Amino Acid Mixture in amount of 29, 37, 46 and 54% of the mean Lysine requirement of 35 mg/kg/d will be given to subjects.
DIETARY_SUPPLEMENTWhite corn meal porridge
A cornmeal porridge including 29, 37, 46 and 54% of the mean Lysine requirement of 35 mg/kg/d will be given to subjects.
Sponsors
The Hospital for Sick Children
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
HEALTH_SERVICES_RESEARCH
Masking
NONE
Eligibility
Sex/Gender
MALE
Age
20 Years to 49 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy Adult males (20-49 years old)
Exclusion criteria
* A history of recent weight loss or illness * Use of any medication at the time of entry into the study
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Metabolic Availability of Lysine | Baseline | 3 breath samples will be taken to provide a baseline F13CO2 measurement (Level of Phenylalanine oxidation). Breath samples will be collected and analyzed for 13CO2 enrichment. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Metabolic Availability of Lysine | 15 mins after intake of the fourth hourly meal (before tracer infusion) | 3 breath samples will be taken to provide a baseline F13CO2 measurement (Level of Phenylalanine oxidation). Breath samples will be collected and analyzed for 13CO2 enrichment. |
Other
| Measure | Time frame | Description |
|---|---|---|
| Metabolic availability of Lysine | 30 mins after intake of the fourth hourly meal (before tracer infusion) | 3 breath samples will be taken to provide a baseline F13CO2 measurement (Level of Phenylalanine oxidation). Breath samples will be collected and analyzed for 13CO2 enrichment. |
| Metabolic Availability of Lysine | 45 mins after intake of the fourth hourly meal (before tracer infusion) | 3 breath samples will be taken to provide a baseline F13CO2 measurement (Level of Phenylalanine oxidation). Breath samples will be collected and analyzed for 13CO2 enrichment. |
Countries
Canada
Outcome results
None listed