Nonalcoholic Fatty Liver Disease, Obesity
Conditions
Keywords
Obesity, Nonalcoholic fatty liver disease, High fructose corn syrup
Brief summary
The purpose of this research study is to learn more about how high fructose corn syrup, a sugar used to sweeten drinks and foods, affects metabolism in obese persons with and without nonalcoholic fatty liver disease. Eligible participants will be studied before and after eating a diet high in high fructose corn syrup or a standard diet (low in high fructose corn syrup) for four weeks.
Interventions
BEHAVIORALHigh fructose corn syrup diet
Subjects will consume a high fructose corn syrup diet for 4 weeks. The food will be prepared by the bio-nutrition kitchen, and meals will be picked up every 3-4 days.
BEHAVIORALStandard diet (low in high fructose corn syrup)
Subjects will consume a standard diet (low in high fructose corn syrup) for 4 weeks. The food will be prepared by the bio-nutrition kitchen, and meals will be picked up every 3-4 days.
Sponsors
Washington University School of Medicine
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
DIAGNOSTIC
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
* IHTG \>6.5% or \<5% * BMI 30.0-45.0 kg/m² * Sedentary * Consume \<10% calories from high fructose corn syrup * Weight stable for previous 3 months
Exclusion criteria
* IHTG 5.1-7.9% * Children * Adults over 65 years * Pregnant * Lactating * Exercise \>1.5 hours/week * Hepatitis B or C * Diabetes * History of liver disease * History of alcohol abuse * Severe hypertriglyceridemia (\>300 mg/dl) * Smokers * Anemia (Hemoglobin \<10mg/dl) * Not weight stable
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in intrahepatic triglyceride (IHTG) content | 4 weeks | Determine the effect of 4-week isocaloric diet high in high fructose corn syrup (HFCS) on IHTG content measured by magnetic resonance spectroscopy (MRS). |
| Hepatic lipid metabolism | 4 weeks | Determine the effect of 4-week isocaloric diet high in HFCS on hepatic lipid metabolism \[de novo lipogenesis, very low density lipoprotein-triglyceride (VLDL-TG) and very low density lipoprotein-Apolipoprotein B100 (VLDL-ApoB) secretion rates\] by using stable isotope tracer methods. |
| Multi-organ insulin sensitivity | 4 weeks | Determine the effect of 4-week isocaloric diet high in HFCS on multi-organ insulin sensitivity by using a one stage hyperinsulinemic euglycemic clamp procedure in conjunction with muscle and adipose tissue biopsies. |
| Uric acid metabolism | 4 weeks | Determine the effect of 4-week isocaloric diet high in HFCS on uric acid metabolism by measuring post-prandial serum uric acid concentrations and using stable isotope tracer method. |
Countries
United States
Outcome results
None listed