Periodontal Disease
Conditions
Brief summary
aggressive periodontitis is an inflammatory disease which damage the teeth supporting structures mostly in young patients. and has genetic basis. a specific bacteria: Aggregatibacter actinomycetemcomitans (Aa) is associated with this disease, and found to damage leukocytes by using a specific leukotoxin. in the research the investigators are aiming to find molecular and immunological basis to aggressive periodontitis.
Interventions
OTHERblood extraction
6 ml blood extraction from each patients in all 3 arms of the study
Sponsors
Polak David
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
* patients in periodontal department * healthy * diagnosed with chronic/aggressive periodontal disease * interested in participating the research
Exclusion criteria
* diagnosed with diabetes, heart disease, immunosuppression, thrombocytopenia, clotting enzyme deficiency. * using alcohol, or drugs * pregnancy
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| flow cytometry | 12 hours after blood extraction | the investigators use flow cytometry technique in order to determine the influence of gingival bacteria on the leukocytes, after culturing the two together. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| microscopy observation | 12 hours after blood extraction | the investigators use microscopy in order to determine the influence of gingival bacteria on the leukocytes, after culturing the two together |
Countries
Israel
Contacts
Primary ContactDavid Polak, DMD, PhD
Backup ContactHadas Lemberg, PhD
Outcome results
None listed