Non- Essential Amino Acid Requirements and Metabolism in Humans

Application of Indicator Amino Acid Oxidation Technique to Determine Non-essential Amino Acids/Nitrogen Requirements in Adult Humans

Status

Completed

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT02009917

Enrollment

Registered

2013-12-12

Start date

2013-10-31

Completion date

2015-04-30

Last updated

2017-07-18

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Healthy Adult Males

Keywords

Indicator Amino Acid Oxidation, Non essential Amino acids, Protein, Amino acid

Brief summary

Protein is a major structural component of all cells in the body. The nutritional importance of protein is because of their amino acids. 9 are called essential and 11 are called non-essential, based on whether we need to get them from diet. This classification is based on studies done using older techniques. Recent studies suggest that these so called non-essential amino acids play important roles in our body's health. For the first time, this proposal defines an experimental design to examine non- essential amino acid/nitrogen requirements and metabolism in humans using the Indicator Amino Acid Oxidation (IAAO) technique. The objective of the current study is to examine the application of IAAO technique to determine the non-essential amino acid/nitrogen requirement in adult humans.

Interventions

None listed

Study design

Observational model

COHORT

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

MALE

Age

20 Years to 40 Years

Healthy volunteers

Yes

Inclusion criteria

* 20-40 years of age * Male * Classified as normal body weight based on body mass index (BMI) 18.5-25kg/m2 * Free of any concurrent illness

Exclusion criteria

* Men not in good health or have a metabolic, neurological, genetic or immune disorder including diabetes and hypertension * Men who are classified as underweight(\<18.5kg/m2), overweight(25-30kg/m2) or obese (\>30kg/m2) using the BMI classification) * Men who are allergic to milk, eggs and egg protein

Design outcomes

Primary

Measure	Time frame	Description
13 Carbon dioxide production	8 hours (1study day)	Urine and breath samples will be collected to measure the rate of oxidation of expired breath and flux by enrichment of urine.

Countries

Canada

Outcome results