Obesity, Type 2 Diabetes Mellitus
Conditions
Brief summary
In this placebo controlled, double-blind, randomized crossover study the investigators will investigate in overweight/obese healthy male volunteers whether inulin administration will increase intestinal SCFA production, thereby investigating whether this will lead to acute metabolic effects.
Detailed description
Gut microbiota is being increasingly recognized as an important factor in fat distribution, insulin sensitivity and glucose and lipid metabolism. Accordingly, the intestinal microbiota could play an important role in the development of obesity and type 2 diabetes mellitus. The role of gut-derived short-chain fatty acids (SCFA), the formation of which is enhanced by microbial fermentation of fibre, is still controversial. At the present time, our understanding of the effects of SCFA on human metabolism (in gut or systemically) is still limited. The investigators hypothesize that the ingested prebiotic inulin is fermented into SCFA and these SCFA have metabolic effects.
Interventions
DIETARY_SUPPLEMENT13C inulin
Oral 13C inulin
OTHERPlacebo
Oral maltodextrin
Sponsors
Top Institute Food and Nutrition
Maastricht University Medical Center
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
PREVENTION
Masking
DOUBLE (Subject, Investigator)
Eligibility
Sex/Gender
MALE
Age
20 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
* Overweight, obese men
Exclusion criteria
* athletes * diabetes mellitus
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Fat oxidation, energy expenditure | 1 study day for each administration | Fat oxidation and energy expenditure are measured after inulin ingestion |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Hormones that influence substrate and energy metabolism | 1 study day for each administration | Insulin, GLP-1, PYY will be measured in plasma after inulin ingestion |
| Circulating metabolites | 1 study day for each administration | Glucose, FFA, TG will be measured in plasma after inulin ingestion |
| Appetite VAS scoring | 1 study day for each administration | Visual Analogue Scale for hunger and appetite are completed after inulin ingestion |
| SCFAs | 1 study day for each administration | SCFA content (acetate, butyrate and propionate, unlabeled and 13C-labeled) are measured after inulin ingestion in plasma, faeces |
| Breath sampling 13CO2 | 1 study day for each administration | In breath samples 13CO2 will be measured after inulin ingestion |
Countries
Netherlands
Outcome results
None listed