Obesity, Overweight
Conditions
Keywords
Lifestyle intervention, Body weight, Body composition, Weight loss maintenance, Physical activity
Brief summary
In Denmark and the western world, there is an increasing prevalence of obesity probably due to a combination of inadequate daily physical activity and a high energy intake. One approach to achieve weight loss and change life style is to participate in an intensive supervised prolonged life style modification course. The immediate effect is often positive, but over time the overall effect is limited as the majority will not maintain weight loss and a changed life style. The purpose of this study is therefore to characterize the physiological factors that determine/influence the capacity to maintain weight loss and a healthy lifestyle after a prolonged lifestyle intervention. We have the following research questions: 1. Are there physiological traits and characteristics that mediate better adherence to lifestyle changes and weight loss? 2. Does the adaptation in muscle oxidative capacity after lifestyle intervention predict success in maintaining weight loss and lifestyle changes 3, 12 and 39 months after intervention? 3. How does gender and age influence the capacity to maintain the lifestyle induced adaptation in muscle and adipose tissue and maintaining weight loss? The study design is a longitudinal intervention and will be based on a lifestyle intervention, as it is practiced in a real life setting at Ubberup folk high school.
Interventions
BEHAVIORALIntensive Lifestyle Intervention
Sponsors
Ubberup Folk High School
University of Copenhagen
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
PREVENTION
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
* Participants at Ubberup Folk High School
Exclusion criteria
* Previous Gastric Bypass Surgery or Gastric Banding * BMI\<25
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Total body weight | Changes from baseline at 3, 6, 15 and 39 months |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Anthropometry | Changes from baseline at 3, 6, 15 and 39 months | Body composition measured by dual x-ray absorptiometry or bio-impedance, height, weight, waist circumference. |
| Maximal Oxygen Consumption | Changes from baseline at 3, 6, 15 and 39 months | — |
| Maximal fat oxidation during exercise | Changes from baseline at 3, 6, 15 and 39 months | Respiratory Exchange Ratio (RER) is measured and maximal fat oxidation is calculated. |
Other
| Measure | Time frame | Description |
|---|---|---|
| Mitochondria respiration and efficiency | Changes from baseline at 3, 6, 15 and 39 months | Mitochondria respiration and efficiency is measured by Oreboros Oxygraph. |
| Blood and plasma substrates, hormones and metabolites | Changes from baseline at 3, 6, 15 and 39 months | HbA1c, insulin, leptin, glucose, adiponectin, thyroid stimulating hormone (TSH), ghrelin |
| Muscle lipid composition | Changes from baseline at 3, 6, 15 and 39 months | Muscle lipid composition will be analysed using a lipidomics approach. |
| Expression of muscle lipases | Changes from baseline at 3, 6, 15 and 39 months | Western blot analysis will be applied to analyse expression of muscle lipases; hormone sensitive lipase (HSL), adipose triglyceride lipase (ATGL) and lipoprotein lipase (LPL) as well as muscle PAT proteins; PLIN 2 (ADRP), PLIN 3 (TIP47) and PLIN 5 (OXPAT) |
| Intramuscular lipid droplet content and size, glycogen content and ceramide content and location | Changes from baseline at 3, 6, 15 and 39 months | Intramuscular lipid droplet content and size, glycogen content and ceramide content and location will be quantified by immunohistochemical staining of serial cryo-sections of muscle tissue samples that are visualized by confocal microscopy |
| Adipose tissue macrophage inflammation and cytokine production | Changes from baseline at 3, 6, 15 and 39 months | — |
| Motivation for lifestyle changes and sociological factors involved | Changes from baseline at 3, 6, 15 and 39 months | Questionnaires |
| Adherence to physical activity | Changes from baseline at 3, 6, 15 and 39 months | International Physical Activity Questionaire (IPAQ) to determine adherence to physical activity. Furthermore we will use diary and ActiHeart (accelerometer). |
| Adherence to healthy diet | Changes from baseline at 3, 6, 15 and 39 months | Questionnaire (24hrs dietary recall) and diary to determine adherence to a healthy diet. |
Countries
Denmark
Outcome results
None listed