Embryo Hypoxia
Conditions
Keywords
low oxygen tension, embryo quality
Brief summary
To investigate the effect of low oxygen tension during in vitro culture in terms of embryo quality and outcomes of pregnancy in vitro fertilization/ Intracytoplasmic sperm injection
Detailed description
The oocytes will be divided into two parts(two groups) form one patient. The two groups of oocytes will be cultured seperately in the incubator with the concentrations of 5% CO2,6%O2,89% N2(low oxygen concentration) verse 6% CO2,20% O2,74% N2(in atmosphere). A series of observational measurement(embryo quality and outcomes of pregnancy) will be performed.
Interventions
OTHER5% oxygen concentration
the oocytes and embryos are cultured in the incubator with concentrations of 6% CO2,5%O2, and 89% N2
Sponsors
Shanxi Provincial Maternity and Children's Hospital
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
NONE
Eligibility
Sex/Gender
FEMALE
Healthy volunteers
No
Inclusion criteria
* Fresh cycles of in vitro fertilization/intracytoplasmic sperm injection and emrbyo transfer (IVF/ICSI-ET) * ≥ 10 oocytes after oocyte retrieval
Exclusion criteria
* ≥ 3 transfer or oocyte retrieval cycles * PGD/PGS cycles * half-ICSI/rescure ICSI * fluid in the uterine cavity, abnormal endometrium, uterine abnormalities,pelvic inflammation * acute maternal problems. * One or both spouses have an abnormal karyotype (including polymorphism)
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| embryo quality | up to 6 days after fertilization | Embryo morphology was assessed on day 2, day 3 day 4 day 5 day and 6 by considering the number of blastomeres, symmetry and granularity of blastomeres, type and percentage of fragmentation, presence of multinucleated blastomeres, and degree of compaction. Gardner's blastocyst scoring system is used for blastocyst assessment |
Countries
China
Contacts
Primary ContactJuanzi Shi, Doctor
Outcome results
None listed