Polycythemia Vera, Essential Thrombocythemia
Conditions
Keywords
Tissue banking
Brief summary
The purpose of the study is to isolate and characterize stem cells of patients with Polycythemia Vera (PV) and Essential Thrombocythemia (ET) to find out why these cells are not working the way they should be and why they seem to be sensitive to regulatory factors in the blood, such as clotting.
Detailed description
Specific Aim 1: Assess the effects of RG7112 on MPN HSC/HPC p53 levels and study how it affects MPN stem cell function. Specific Aim 2. Assess the effects of a combination of low doses of IFNα and RG7112 on MPN HSC/HPC.
Interventions
PROCEDUREBlood specimen
4 green top tube 40 cc of blood draw
Sponsors
The Leukemia and Lymphoma Society
Icahn School of Medicine at Mount Sinai
Study design
Observational model
OTHER
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Healthy volunteers
No
Inclusion criteria
* polycythemia vera or essential thrombocythemia * agree to give blood for study
Exclusion criteria
* none
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Stem cell function | at one time study visit | Assess the effects of RG7112 on MPN HSC/HPC p53 levels and study how it affects MPN stem cell function. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| CD34+ cells assays | at one time study visit | Assess the effects of a combination of low doses of IFNα and RG7112 on MPN HSC/HPC. After treatment with RG7112 and IFNα at the various doses, p53, MDM2, MDMX, phosphor-p38, and phosphor-STAT1 levels in MPN CD34+ cells will be measured using monoclonal antibody staining and flow cytometric and immunofluorescence analyses. |
Countries
United States
Outcome results
None listed