Atopic Dermatitis, Eczema
Conditions
Keywords
Autologous microbiome transplant, Bacteria transplant, Treatment for atopic dermatitis
Brief summary
Unlike healthy control skin, the skin of patients with atopic dermatitis (AD) is frequently colonized by Staphylococcus aureus (S. aureus), putting these patients at increased risk of S. aureus skin infections. In addition, research in the investigator's lab has shown that these patients have fewer protective antimicrobial Staphylococcal species such as Staphylococcal epidermidis (S. epidermidis) known to produce antimicrobial peptides that play a role in protecting the skin from invading pathogens. In this study, the investigator will attempt to decrease S. aureus colonization and increase colonization by protective Staph species. First the investigator will culture the bacteria on subjects' lesional AD skin. The investigator will selectively grow the subject's antimicrobial Staph colonies and place them into a base moisturizer. The moisturizer plus bacteria will be applied to both of the subject's arms. Prior to applying this, though, one arm will first be pre-treated with an antimicrobial regiment of Dial liquid antibacterial soap and alcohol. We will then compare the abundance of antimicrobial Staph species on each subject's arms 24 hours later to determine whether the pre-treatment regimen increased survival of the transplanted antimicrobial Staph species. The investigator expects that the arm pre-treated with the antimicrobial regimen will have more antimicrobial Staph species at this time point.
Interventions
DRUGPre-Treatment with Dial liquid antibacterial soap
This arm will be pre-treated with Dial liquid antibacterial soap and alcohol prior to the autologous microbiome transplant.
OTHERControl
Sponsors
University of California, San Diego
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
SINGLE (Investigator)
Eligibility
Sex/Gender
ALL
Age
18 Years to 80 Years
Healthy volunteers
No
Inclusion criteria
* Male or female subjects who are not pregnant or lactating * 18-80 years of age * Diagnosis of atopic dermatitis for at least 6 months using the Hanifin and Rajka Diagnostic Criteria for atopic dermatitis * Presence of lesional atopic dermatitis skin in both antecubital fossae * Positive S. aureus colonization based on results of a skin culture taken from one of their AD-affected antecubital fossae during the screening visit
Exclusion criteria
* Use of any topical AD treatments (including topical steroids, topical calcineurin inhibitors) to either arm within one week of either screening visit * Use of any oral/systemic AD therapies (antihistamines, steroids) within 28 days of either screening visit * Severe AD that would worsen significantly from holding a participant's usual topical/oral AD medications for the time periods required in the inclusion/
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Abundance of antimicrobial Staph colonies | 24-hours post-transplant | Quantitative washes will be used to measure the abundance of antimicrobial Staph colonies on both the pre-treated and the not pre-treated arms 24 hours after the microbial transplant |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Adverse events | 24 hours post-transplant | All adverse events associated with use of the transplant cream will be recorded |
Countries
United States
Outcome results
None listed