Diabetes Type 2
Conditions
Brief summary
Type 2 diabetes is characterized by increased insulin resistance and impaired insulin secretion. In addition, type 2 diabetes is associated with low grade inflammation indicated by increased levels of proinflammatory cytokines such as TNF-α. TNF-α has previously been shown to impair peripheral insulin signaling in vitro and in vivo. However, it is unclear whether TNF-α may also affect endogenous glucose production (EGP) during fasting and glucose stimulated insulin secretion (GSIS) in vivo. We hypothesized that low dose TNF-α would increase EGP and attenuate GSIS. Recombinant human TNF-α or placebo was infused in healthy, non-obese and non-diabetic young men (n=10) during a 4-hour basal period followed by an intravenous glucose tolerance test (IVGTT).
Interventions
OTHERLow-dose TNF-alpha
Sponsors
Copenhagen Muscle Research Centre, Rigshospitalet, Copenhagen, Denmark
University of Copenhagen
Rigshospitalet, Denmark
Study design
Primary purpose
BASIC_SCIENCE
Eligibility
Sex/Gender
MALE
Age
20 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy men * Age 20-35
Exclusion criteria
* obesity (BMI\>25) * diabetes * chronic disease
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Beta-cell function | Measurements at 0h, 1h, 2h, 3h and 4h during basal period. After glucose bolus infusion, measurements every 1 min for the first 30 min and every 3 mins for the next hour. Last measurement 1,5 hours post- IVGTT | Insulin levels (pg/ml) in blood measured continually before and after Intravenous glucose tolerance test. Glucose bolus infused in healthy young men after a 4 hour basal period. Subsequent measurements og insulin and glucose levels in peripheral blood |
Countries
Denmark
Outcome results
None listed