Evaluation of Granulosa Cells Function After IVF Protocols

In Vitro Evaluation of Luteinized Granulosa Cells After Being Submitted to in Vitro Fertilization Protocols With Gonadotropin-Releasing Hormone (GnRH) Agonist and GnRH Antagonist

Status

UNKNOWN

Phases

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT01946022

Enrollment

Registered

2013-09-19

Start date

2013-09-30

Completion date

Unknown

Last updated

2013-11-07

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Infertility, In Vitro Fertilization Protocols

Keywords

infertility, ovulation induction, luteal phase, granulosa cells function, GnRH agonist and antagonist, HMG, FSHr

Brief summary

This study is intended to evaluate ovarian cells after being submitted do different in vitro fertilization protocols. The investigators will analyze hormones secretion and intracellular mechanisms of these cells comparing the GnRH agonist protocol and the GnRH antagonist protocol.

Interventions

DRUGrandomization between the two GnRH analogues (agonist and antagonist)

Study design

Allocation

RANDOMIZED

Intervention model

PARALLEL

Primary purpose

BASIC_SCIENCE

Masking

NONE

Eligibility

Sex/Gender

FEMALE

Age

20 Years to 39 Years

Healthy volunteers

Yes

Inclusion criteria

* Patient age limit: 39 years * Follicle Stimulating Hormone (FSH) \< 10 micrometer (mU) / Liter * Antral follicular count \> 12 * Regular menses (25 to 35 days) * More than 3 oocytes after ovarian punction * Anti Mullerian between 1 e 3 ng/ml

Exclusion criteria

* Patients submitted to intracytoplasmatic sperm injection (ICSI) * Ovarian mass or cyst * Clinical / endocrine pathologies

Design outcomes

Primary

Measure	Time frame	Description
Evaluation of steroid secretion and enzyme expression in in vitro luteinized granulosa cells	after 48h in media culture	evaluation of granulosa cells hormone secretion and enzyme expression in media culture 48 hours after ovarian punction
Number of retrieved oocytes	After the ovarian punction	Immediately after the ovarian punction the number of retrieved oocytes will be evaluated

Secondary

Measure	Time frame	Description
number of embryos	3 to 5 days after ovarian punction	the embryologist will verify the number of embryos after 3 to 5 days from the ovarian punction
Subgroup analyses comparing FSH versus HMG protocols	after ovarian punction	It will be verified the differences between the two types of gonadotrophins in ovarian stimulation regarding: units of gonadotrophins used, number of retrieved oocytes,number of embryos and embryo score

Countries

Brazil

Contacts

Primary ContactRita d Chapon

rchapon@gmail.com5192028288

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026