Exercise-induced Changes in DNA-methylation

The Effect of Exercise on Maximal Aerobic Capacity, DNA-methylation and Gene Expression in Healthy Elderly Men

Status

Terminated

Phases

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT01931072

Enrollment

Registered

2013-08-29

Start date

2012-08-31

Completion date

2013-12-31

Last updated

2019-05-20

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Healthy

Keywords

Exercise, High-intensity interval training, Moderate training, Healthy elderly, Skeletal muscle, DNA-methylation, Gene expression, subjects

Brief summary

The purpose of the study was to investigate whether regular moderate- or high-intensity interval training can change the DNA methylation in a long-lasting state, and whether these changes affect the gene expression. In addition, the investigators will determine whether the intensity of the training could be an affecting factor of the possible DNA-methylation change as well. The null hypothesis was that no such exercise-induced changes occur.

Interventions

BEHAVIORALHigh-intensity interval training

Interval training 3 times a week for 8 weeks

BEHAVIORALModerate training

Moderate training 3 times a week for 8 weeks

Study design

Allocation

RANDOMIZED

Intervention model

PARALLEL

Primary purpose

BASIC_SCIENCE

Masking

NONE

Eligibility

Sex/Gender

MALE

Age

70 Years to 75 Years

Healthy volunteers

Yes

Inclusion criteria

* Male * 70-75 years old * Signed written informed consent

Exclusion criteria

* Incompatible with exercise * Known cardiac disease * Active cancer * Significant pulmonary disease * Uncontrolled hypertension * Use of blood thinning medicine (except Albyl-E) * Drug abuse * Alcohol abuse * Finishing less than 80% of the exercise sessions (this complies to the training groups)

Design outcomes

Primary

Measure	Time frame	Description
Gene Expression	8 weeks	—
DNA-methylation	8 weeks	From the skeletal muscle biopsies RNA and DNA was isolated, DNA was further bi-sulfite converted and DNA-methylation was analyzed using the Illumina HumanMethylation450 BeadChip. The RNA was further used for gene expression analyses by real-time RTqPCR.

Secondary

Measure	Time frame	Description
Maximal Aerobic Capacity	8 weeks	measured on treadmill at baseline and after eight weeks, also biopsies from Vastus lateralis were sampled.

Countries

Norway

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026