Android Obesity, Metabolic Parameters and Adipose Tissue (AT) Gene Expression
Conditions
Brief summary
o determine effects of hula-hooping on android obesity, metabolic parameters and adipose tissue (AT) gene expression.
Interventions
OTHERExercise
Sponsors
University of Helsinki
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 70 Years
Healthy volunteers
Yes
Inclusion criteria
* age between 18 and 70 years * central adiposity as measured by the waist circumference (\>80 cm in females and \>94 cm in males) (9) * ability to hula-hoop for a minimum of 2 minutes and to have the necessary space available for this activity either at home or at work * ability to communicate meaningfully with the investigator and legal competence to provide written informed consent
Exclusion criteria
* clinical or biochemical evidence of disease other than obesity as judged by history, physical examination and standard laboratory tests * excessive use of alcohol, i.e., over 20 g/day * use of medications known to affect glucose or lipid metabolism and * pregnancy or lactation
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Android obesity | 6weeks | The primary objective was to evaluate the change in the % fat in the android region as measured by DEXA during 6 weeks of HULA as compared to WALK |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| metabolic parameters | 6weeks | The secondary objectives included the comparison of differences in waist circumference (cm), relative and absolute trunk and limb muscle mass as measured by DEXA (% and kg), fasting plasma glucose (mmol/l), fasting serum insulin (mU/l), liver enzymes (U/l), fasting plasma LDL and HDL cholesterol and triglycerides (mmol/l). Gene expression was analyzed in adipose tissue biopsies collected before and after each exercise intervention. |
Other
| Measure | Time frame | Description |
|---|---|---|
| adipose tissue inflammation | 6weeks | To determine the effects of HULA compared to WALK on adipose tissue inflammation, a needle aspiration biopsy of abdominal s.c. adipose tissue was taken under local anesthesia with lidocaine before and after each exercise intervention. The adipose tissue sample was immediately frozen in liquid nitrogen and then stored at -80°C until analysis. Total RNA from s.c. AT was isolated.Relative quantification for the genes of interest (see list below) was carried out using normalization to the 36B4 and β-actin mRNA values. ABCA1 Adiponectin ANGPTL8 CHOP CD4 CD8 CD68 ETFDH FOXP3 GLUT4 MCP-1 MRC-1 PGC-1α VEGF-A β-actin |
Countries
Finland
Outcome results
None listed