MACS Technique Efficacy
Conditions
Keywords
MACS, time-lapse, embryo, spermatozoid, sperm
Brief summary
The MACS technique (magnetic-activated cell sorting) is based on the usage of microbeads, magnetic particles with a diameter of 35 nanometers joined to the compound annexin V. Annexin V is a protein with a high affinity for the molecule phosphatidylserine. This system allow us to eliminate those spermatozoids which have begun the apoptosis and have phosphatidylserine residues on their membrane surface. Using this technique, the apoptotic spermatozoids will join the microbeads and consequently be retained in a column applying a magnetic field. Recent studies show the benefits of spermatozoid selection using MACS and the improvement in mobility, viability, DNA fragmentation and fertilization potential. It has also been observed an improvement in the embryo cells division rate, clinical pregnancy rates and spermatozoid survival after thawing. However, it is necessary to perform new clinical studies in order to evaluate the real potential of this technology and its safety. The purpose of this study is to evaluate the effect of the MACS technique on the clinical results of good-prognosis patients who are going through IVF/ICSI treatments in order to achieve a pregnancy. The hypothesis of this study is that the application of the MACS technique in assisted reproduction treatments is associated with a better embryo quality and pregnancy rates.
Interventions
OTHERMACS
The sperm will be subjected to capacitation through a density gradient together with the MACS technique in order to select the non-apoptotic spermatozoids.
OTHERNO MACS
The sperm capacitation will be performed through a density gradient. The MACS technique will not be applied.
Sponsors
Vida Recoletas Sevilla
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
OTHER
Masking
DOUBLE (Subject, Caregiver)
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 38 Years
Healthy volunteers
No
Inclusion criteria
* \<38 years * \>10 follicles with a diameter of ≥10 mm the day before the follicular puncture
Exclusion criteria
* Patients subject to preimplantation genetic diagnosis treatments
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Clinical pregnancy rate | 14 months |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Pregnancy rate | 14 months | — |
| Embryo quality | 14 months | According to ASEBIR criteria |
| Implantation rate | 14 months | — |
Countries
Spain
Outcome results
None listed