Muscle Loss
Conditions
Brief summary
The primary aim of this study is to determine the effect of creatine supplementation on muscle mass loss during short-term immobilisation in healthy, young people.
Detailed description
Seven days of one-legged knee immobilisation in young subjects with creatine or Placebo supplementation. A randomly selected leg is immobilized for seven days. Muscle mass and strength will be determined and muscle biopsies will be collected before and immediately after immobilisation. A week after the cast is removed we will assess any differences in recovery between intervention groups.
Interventions
OTHERPlacebo supplementation
Seven days of one-legged knee immobilisation with placebo supplementation
Seven days of one-legged knee immobilisation with creatine supplementation.
Sponsors
Maastricht University Medical Center
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
PREVENTION
Masking
TRIPLE (Subject, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
MALE
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* Male * Aged from 18-35 years * 18.5 \< BMI \< 30 kg/m2
Exclusion criteria
* (Family) history of thrombosis * Smoking * Recent surgery * Performing regular resistance training more than once per week in the past year * Any back/leg/knee/shoulder complaints which may interfere with the use of crutches * Current systemic use of corticosteroids, growth hormone, testosterone, immunosuppressants or insulin * All co-morbidities interacting with mobility and muscle metabolism of the lower limbs (e.g. arthritis, spasticity/rigidity, all neurological disorders and paralysis) * Use of anti-coagulants * Pre-existing renal disease or those with a potential risk for renal dysfunction (diabetes, hypertension, reduced glomerular filtration rate)
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| change in quadriceps muscle cross-sectional area (CSA) | Before and after 1 week of immobilisation, and after 1 week of recovery | immediately before and after 1 week of immobilisation, and after 1 week recovery, quadriceps CSA will be assessed |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| change in muscle fiber type specific CSA | Before and after 1 week of immobilisation, and after 1 week of recovery | immediately before and after 1 week of immobilization, and after 1 week of natural recovery |
| change in muscle strength | before and after 1 week of immobilisation, and after 1 week of recovery | immediately before and after 1 week of immobilization, and after 1 week of natural recovery, one-repetition maximum (1RM) muscle strength will be assessed |
Countries
Netherlands
Outcome results
None listed