Optimization of Time-of-Flight Mass Cytometry (CyTOF) Analysis for Evaluation of Immune Changes Following Surgery

Detection of Immune Changes as a Result of Surgical Trauma in Human Back Surgery Subjects: Optimization of CyTOF Analysis

Status

Completed

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT01882569

Enrollment

Registered

2013-06-20

Start date

2012-05-31

Completion date

2014-09-30

Last updated

2016-05-04

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Surgical Trauma

Brief summary

Surgical trauma triggers a massive inflammatory response. Over time, both the innate and adaptive branches of the immune system are affected by surgical trauma. The purpose of this study is to use a single cell flow cytometry approach to characterize the cellular and molecular mechanisms involved in the inflammatory response to surgical trauma.

Interventions

None listed

Study design

Observational model

COHORT

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

ALL

Age

18 Years to 90 Years

Healthy volunteers

Inclusion criteria

1. Ages 18 - 90 2. Men and women 3. Undergo back surgery

Exclusion criteria

1. Any systemic disease that might compromise the immune system (e.g. lupus) 2. Current diagnosis of cancer 3. Any condition that, in the opinion of the investigator, might compromise the integrity of the study or safety of the participant. 4. Unwilling to sign informed consent

Design outcomes

Primary

Measure	Time frame	Description
Numerical (cell frequencies) and functional (phosphorylation of signaling proteins and transcription factors) changes of all circulating immune cells	Immediately before to surgery, 1 hour after surgery, 24 hours after surgery, and day of hospital discharge	This is an exploratory study.

Countries

United States

Outcome results