Natural Cycle in Vitro Fertilization (IVF)
Conditions
Keywords
Natural, IVF Cycle
Brief summary
Natural IVF study patients will undergo an IVF cycle with trigger shots of Lupron & hCG. Patients will not be given any other stimulating medications during the cycle. They will have 2 uterine aspirations and an endometrial biopsy at designated times. The first uterine aspiration will be randomized to one of four days (day before retrieval, day of retrieval, day after retrieval or 2 days after retrieval). The second uterine aspiration and endometrial biopsy will be performed 6 days after retrieval. Any embryo(s) obtained will be biopsied for Comprehensive Chromosome Screening (CCS) and frozen for use in a subsequent thaw cycle.
Interventions
None listed
Sponsors
Reproductive Medicine Associates of New Jersey
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 50 Years
Healthy volunteers
No
Inclusion criteria
* Ovulatory cycles every 39 days or less (natural cycle IVF cannot be performed in someone who does not have cycles) * Must be RMANJ patient and have completed all diagnostic screening at one of our RMANJ locations. All diagnostic screening is NOT covered under the study and must be completed prior to being allowed to start the study cycle.
Exclusion criteria
* Prior enrollment/attempt in this study * Requirement for surgical sperm extraction * Inability to assess ovaries via transvaginal ultrasound * Inability to tolerate vaginal examination
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Endometrial Changes | 1 Year | To examine the endometrium within the natural cycle in order to accurately characterize the differences in multiple aspects of endocrine and paracrine hormonal activity, the transcriptome of cells influencing oocyte maturation and changes in the endometrium. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Aneuploidy Rates | 1 Year | To examine aneuploidy rates in embryos from natural IVF cycles. |
Other
| Measure | Time frame | Description |
|---|---|---|
| Temporal Embryonic Development | 1 Year | To examine the parameters of temporal embryonic development and the transcriptome of granulosa cells and follicular fluid. The embryos will be graded at standard time points to determine whether there are differences in in vitro development. Granulosa cells and follicular fluid will undergo gene expression analysis and compared to the gene expression profiles found in those specimens in stimulated cycles. |
Countries
United States
Outcome results
None listed