Male Infertility, Unexplained Infertility
Conditions
Keywords
Spermatozoa, DNA fragmentation, Swim-up, Density gradient
Brief summary
Sperms are prepared by sperm washing techniques before used in assisted reproductive technologies. Most commonly used sperm preparation methods are the swim up and the density gradient. Recent studies shows that the DNA integrity status of the spermatozoa is related to the success in assisted reproduction techniques. Sperm preparation methods may theoretically cause damage to sperm DNA. Therefore it is important to select the optimum method of sperm preparation causing least sperm DNA damage. Aim of our study is to investigate and compare the effect two different sperm preparation techniques on DNA fragmentation.
Interventions
PROCEDUREswim-up, density gradient sperm preparation techniques
Sperms are prepared by sperm washing techniques before used in assisted reproductive technologies. Most commonly used sperm preparation methods are the swim up and the density gradient. Recent studies shows that the DNA integrity status of the spermatozoa is related to the success in assisted reproduction techniques. Sperm preparation methods may theoretically cause damage sperm DNA. Therefore it is important to select the optimum method of sperm preparation causing least sperm DNA damage. Aim of our study is to investigate and compare the effect two different sperm preparation techniques on DNA fragmentation.
Sponsors
Gazi University
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
DIAGNOSTIC
Masking
NONE
Eligibility
Sex/Gender
MALE
Age
20 Years to 45 Years
Healthy volunteers
No
Inclusion criteria
Male factor subfertile group: Couples of having at least two abnormal sperm analysis according to WHO criteria. Unexplained subfertile group: Couples with normal sperm parameters, plus females with normal ovulatory status and patent fallopian tubes by hysterosalpingography or laparoscopy. No previous IVF or IUI attempts before the study
Exclusion criteria
Severe oligospermia (Sperm count \< 5 Millions/ per ml) Systemic diseases or therapies influencing DNA integrity for male partner
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| The change in the rate of DNA fragmentation of spermatozoa after sperm preparation with either swim-up or density gradient techniques. | DNA fragmentation assay is performed just before and immediately after the sperm preparation is completed. The time frame is within an hour. | The change in the rate of DNA fragmented spermatozoa will be assessed before and immediately after the sperm preparation is completed, because DNA fragmentation of spermatozoa may be increased in time. |
Outcome results
None listed