Muscle Synthesis
Conditions
Keywords
soy, whey, muscle, older, mTOR, sarcopenia
Brief summary
During aging there is a tendency for muscle protein synthesis (growth) to become less efficient and the resulting consequence leads to reduced muscle mass (sarcopenia) which can affect strength and mobility. Protein consumption may be one opportunity to alleviate this problem especially when consumed in appropriate amounts following resistance training. This study is designed to determine if a soy dairy protein blend, shown to be effective in younger adults, will produce beneficial muscle protein synthesis in older healthy adults following resistance exercise.
Interventions
DIETARY_SUPPLEMENTSoy Dairy Protein Blend
DIETARY_SUPPLEMENTPositive Control (Dairy Whey Protein)
Sponsors
DuPont Nutrition and Health
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
OTHER
Masking
QUADRUPLE (Subject, Caregiver, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
MALE
Age
55 Years to 70 Years
Healthy volunteers
Yes
Inclusion criteria
55-70 yr olds Stable Body weight
Exclusion criteria
1. Exercise training (\>2 weekly sessions of moderate to high intensity aerobic or resistance exercise) 2. Significant heart, liver, kidney, blood, respiratory disease or thyroid issues 3. Peripheral vascular disease 4. Orthopedic injury that prohibits participation in the exercise training 5. Diabetes mellitus or other untreated endocrine disease 6. Active cancer (all groups) and history of cancer 7. Acute infectious disease or history of chronic infections (e.g. TB, hepatitis, HIV, herpes) 8. Neurologic Injury or disease 9. Recent (within 6 months) treatment with anabolic steroids, or corticosteroids. 10. Alcohol or drug abuse 11. Tobacco use (smoking or chewing) 12. Malnutrition (BMI \< 20 kg/m2, hypoalbuminemia, and/or hypotransferrinemia) 13. Obesity (BMI \> 30 kg/m2) 14. Low hemoglobin levels (below normal values) 15. Food allergies 16. Taking dietary supplements such as green tea, omega 3 fatty acids, etc. 17. Females
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Fractional Synthetic Rate (Muscle Protein Synthesis) | baseline, 3 hrs, 5 hrs | The fractional synthesis rate (FSR) of mixed muscle proteins will be calculated from the incorporation rate of L-\[ring-13C6\]Phenylalanine into the mixed muscle proteins, and the free-tissue phenylalanine enrichment. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Anti-inflammatory and Anti-oxidant Markers | baseline, 3 hrs, 5 hrs | Proteins will be assessed that are markers of oxidative damage and inflammation (NFkB, IL-1, IL-6) and proteolysis (MuRF, MAFBx). |
| Intracellular Signalling Genes and Proteins | baseline, 3 hrs, 5 hrs | Phosphorylation of Mammalian Target of Rapamycin (mTOR) pathway will be measured using Western blot techniques |
Countries
United States
Outcome results
None listed