Plasmodium Falciparum Malaria
Conditions
Keywords
RDT, malaria, treatment follow up, diagnosis
Brief summary
The aim of the study was to follow clearance of malaria infections and detection of new malaria episodes after initiation of antimalarial treatment in Tanzanian children. For this purpose the investigators used five diagnostic tools, 2 Rapid Diagnostic tests based on Histidine Rich Protein 2(HRP2) and Lactate dehydrogenase(LDH), 2 microscopical methods and one polymerase chain reaction (PCR). The investigators followed the 53 enrolled children during 42 days.
Interventions
DEVICEHRP2 and LDH based rapid diagnostic tests for P.falciparum
Sponsors
Muhimbili University of Health and Allied Sciences
Anders Björkman
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
6 Months to 59 Months
Healthy volunteers
No
Inclusion criteria
* children between 6-59 months * fever (≤37.5 C) or history of fever during the preceding 24 hours * confirmed P.falciparum monoinfection * uncomplicated malaria * parasite density between 2000-250.000/µL * willing/able to comply with the 42 day follow up * informed concent from patient/guardian
Exclusion criteria
* children with history of antimalaria drug intake within the last two weeks * symptoms / signs of severe disease
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Clearance time with five diagnostic tests | 2009 June to 2012 May (up to day 42) | Children were followed up on 9 occasions until day 42. Mean and median time until clearance after initiation of arthemeter-lumefantrine treatment was measured for HRP2 based Rapid diagnostic tests, LDH-based Rapid diagnostic tests, Giemsa and Acridine Orange stained microscopy and real-time PCR. |
| Detection of recurrent infection with HRP2 and LDH-based Rapid diagnostic tests during follow up | 2009 June to 2012 May (up to day 42) | Ability to detect recurrent malaria infection with the HRP2 and LDH-Rapid diagnostic tests during the 42 days of follow up was studied through comparing them with three other diagnostic tests. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Efficiency of P.falciparum specific rapid diagnostic tests | 2009 June to 2012 May (up to day 42) | Efficiency of two rapid diagnostic tests based on two different antigens for diagnosis and treatment follow up among children \<5 years in a moderately high endemic area was determined. Specificity and false positivity rate for HRP2 and LDH based rapid diagnostic tests are calculated against Giemsa stained microscopy and PCR. |
Countries
Tanzania
Outcome results
None listed