Detection Of Monocytes/Macrophages Function And Tim-3 Expression In Septic Patients

Status

Completed

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT01801839

Enrollment

Registered

2013-03-01

Start date

2012-08-31

Completion date

Unknown

Last updated

2016-01-28

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Sepsis, SIRS(Systemic Inflammatory Response Syndrome)

Brief summary

The imbalance between anti- and pro-inflammation often occurs in patients with sepsis. And continuous such imbalance could lead to immunoparalysis, which is characterized by loss of delayed type hypersensitivity, failure of primary infections eradication, and a predisposition of secondary nosocomial infections development. However, in which phase of sepsis does immunoparalysis occur is still unknown. So this study aims to tentatively understand this problem by checking monocytes/macrophages' antigen presentation and cytokine secretion，and by checking monocytes/macrophages' expression of Tim-3 (the T cell transmembrane, immunoglobulin, and mucin-3), which is an inhibitory regulatory molecule, in sepsis patients' peripheral blood mononuclear cells.

Interventions

None listed

Study design

Observational model

CASE_CONTROL

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

ALL

Age

18 Years to No maximum

Healthy volunteers

Yes

Inclusion criteria

* age over 18 years old; * Fulfilled at least two criteria of systemic inflammatory response syndrome (a) core temperature higher than 38 ℃ or lower than 36 ℃ (b)respiratory rate above 20/min, or carbon dioxide partial pressure below 32 mmHg (c) pulse rate above 90/min, and (d) white blood cell count greater than 12,000/μl or lower than \< 4,000/μl or less than 10% of bands. * Clinically suspected infection;

Exclusion criteria

* were under 18 years of age; * were refused by the patients or his/her familiality; * were suffering from acquired immunodeficiency syndrome,viral hepatitis,autoimmune diseases and hematology; * use medicine which can affect immune system,such as Glucocorticoid and Immunosuppressant * died within 24h after being taken into the ICU, or refused to get involved in the study, or gave up treatment during the period of observation

Design outcomes

Primary

Measure	Time frame
systemic inflammatory response to infection with organ dysfunction, hypoperfusion, or hypotension	within 24 hours after enter experimental group

Countries

China

Outcome results