Fructose-induced Intestinal de Novo Lipogenesis

Measure of Intestinal Lipogenesis in Healthy Volunteers and Obese Subjects Before and After Gastroplasty: Modulation by Glucose and Fructose

Status

Completed

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT01792089

Acronym

FIDNL

Enrollment

Registered

2013-02-15

Start date

2013-02-28

Completion date

2016-12-31

Last updated

2019-03-25

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Obesity

Keywords

de novo lipogenesis, fructose, roux-en-Y gastric bypass, post-prandial triglycerides

Brief summary

8 healthy volunteers, 8 pre-bypass obese subjects, 8 subjects between 12-18 months post-bypass, and 8 subjects matched to the post-bypass patients will be studied in two sub-studies Sub-study 1) after ingestion of two or three different of the following test-meals: A)cream and whey protein B)cream, whey protein and fructose + 13C6 fructose C)test meal with cream, whey protein, fructose and glucose + 13C6 fructose Their intestinal de novo lipogenesis will be estimated by measuring 13C-palmitate on triglyceride-rich lipoprotein particles associated with apoB48 in blood Sub-study 2) after ingestion of two or three different of the following test-meals: A)cream labelled with 13C6 palmitic acid and whey protein B))cream labelled with 13C6 palmitic acid, whey protein, fructose and glucose Their intestinal de novo lipogenesis will be estimated by measuring 13C-palmitate on triglyceride-rich lipoprotein particles associated with apoB48 in blood in sub-study 1 Their exogenous lipid absorption kinetics will be assessed by measuring 13C palmitate on triglyceride-rich lipoprotein particles associated with apoB48 in sub-study 2

Interventions

OTHERfat+protein meal

cream+ whey protein

OTHERFat + protein + 13C fructose meal

cream+ whey protein +13C fructose

OTHERFat+protein+13C fructose+glucose meal

Cream + whey protein + glucose:13C fructose mixture

OTHER13C Fat + whey protein

cream labelled with 13C palmitate + whey protein

OTHER13C Fat + whey protein + glucose+ glucose meal

cream labelled with 13C palmitate + whey protein + glucose:fructose mixture

Study design

Observational model

CASE_CONTROL

Time perspective

CROSS_SECTIONAL

Eligibility

Sex/Gender

ALL

Age

25 Years to 50 Years

Healthy volunteers

Yes

Inclusion criteria

* age 18-50 years * sex male or female * less than 2 kg body weight change over the past 3 months subjects

Exclusion criteria

* antidiabetic and hypolipemic drugs * alcohol consumption \>20g/day * severe eating disorders * severe psychological problems * vegetarian diet or other specific diet * consumption of illicit substances * pregnancy * weight gain or weight loss \> 1 kg in the last 3 months

Design outcomes

Primary

Measure	Time frame	Description
Intestinal de novo lipogenesis (sub-study 1)	0-8-hour post-prandial	Measurement of total TG and 13C palmitate in chylomicrons: The sf \>20 fraction of post-prandial blood samples will be prepared, and non-chylomicrons, apoB100-containing lipoprotein will be removes by immuno-affinity chromatography using a specific anti-apoB100 antibody
Postprandial exogenous, labelled palmitate concentration (sub-study 2)	0-8-hour post-prandial	Measurement of total TG and 13C palmitate in chylomicrons of sf \>20 fraction of post-prandial blood samples will be prepared,

Secondary

Measure	Time frame	Description
post-prandial triglyceride profile (sub-studies 1 and 2	0-8 hour post-prandial	Triglyceride will be measured in the chylomicron, VLDL, LDL and HDL fractions of post-prandial blood samples

Countries

Switzerland

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Mar 7, 2026