Contribution of Ethanol on Hydroxytyrosol Formation
Conditions
Keywords
Antioxidants, Central Nervous System Agents
Brief summary
Health benefits for humans derived from low dose intake of ethanol could partially be explained by its interaction with dopamine (DA) oxidative metabolism. Ethanol is expected to induce an increase in the formation of a DA minor metabolite: DOPET (hydroxytyrosol). Hydroxytyrosol is one of the most potent antioxidants present in Mediterranean Diet. The study is aimed at establishing the contribution of ethanol on hydroxytyrosol formation.
Interventions
DIETARY_SUPPLEMENTEthanol
OTHERWater
Sponsors
Fundacion IMIM
Parc de Salut Mar
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
DOUBLE (Subject, Investigator)
Eligibility
Sex/Gender
MALE
Age
18 Years to 55 Years
Healthy volunteers
Yes
Inclusion criteria
* Clinical history and physical examination demonstrating no organic or psychiatric disorders. * The ECG and general blood and urine laboratory tests performed before the study should be within normal ranges. Minor or occasional changes from normal ranges are accepted if, in the investigator's opinion, considering the current state of the art, they are not clinically significant, are not life-threatening for the subjects and do not interfere with the product assessment. These changes and their non-relevance will be justified in writing specifically. * The body mass index (BMI=weigh/height2) will range from 19 to 27 kg/m2, and the weight from 50 to 110 kg. * Understanding and accepting the study procedures and signing the informed consent.
Exclusion criteria
* Not meeting the inclusion criteria. * History or clinical evidence of alcoholism, drug abuse, or regular use of psychoactive drugs. * Having suffered any organic disease or major surgery in the three months prior to the study start. * History of psychiatric disorders. * Smokers of more than 20 cigarettes per day. * Taking more than 30 g of alcohol a day * Regular use of any drug in the month prior to the study sessions.The treatment with single or limited doses of symptomatic medicinal products in the week prior to the study sessions will not be a reason for exclusion if it is calculated that it has been cleared completely the day of the experimental session. * Blood donation 8 weeks before or participation in other clinical trials with drugs in the previous 12 weeks. * Subjects with intolerance or adverse reactions to ethanol * History or clinical evidence of gastrointestinal, liver, renal or other disorders which may lead to suspecting a disorder in drug absorption, distribution, metabolism or excretion, or that suggest gastrointestinal irritation due to drugs. * Subjects unable to understand the nature, consequences of the study and the procedures requested to be followed. * Subjects with positive serology to Hepatitis B, C or HIV. To be eligible, the subjects must agree to follow a diet free from ethanol and olive oil in the 72 hours prior to the start of each session and until the end of the study.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Hydroxytyrosol biological fluids concentrations | 0-24h after administration | Hydroxytyrosol concentrations in plasma and urine |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Subjective drunkenness | 0-6h after administration | Ethanol subjective effects measured using a visual analog scale |
| Vital signs | 0-6h after administration | Blood pressure, oral temperature and heart rate |
| Ethanol concentration | 0-6h after administration | Ethanol blood concentrations |
| Ethanol metabolites concentrations | 0-24h after administration | Ethanol metabolites recovery in urine |
| Dopamine metabolites concentrations | 0-24h after administration | Dopamine metabolites recovery in urine |
Countries
Spain
Outcome results
None listed