Chromosomal Aneuploidies, Single Gene Desease
Conditions
Keywords
Blastocoele fluid, Human DNA, molecular diagnosis
Brief summary
The objective will focus on the characterization of DNA fragments present in Blastocoele fluid, and the evaluation of these fragments as potential target for Preimplantation Genetic Diagnosis. To reach this goal, real-time PCR, Whole Genome Amplification techniques and -subsequently- Next Generation Sequencing and aCGH approaches will be used.
Interventions
OTHERblastocoele aspiration
Sponsors
Cervesi Hospital, Cattolica, Italy
Study design
Observational model
COHORT
Time perspective
OTHER
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* IVF patient with supernumerary embryos to vitrify at blastocyst stage
Exclusion criteria
* IVF patient without blastocyst formation
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Number of samples with DNA in blastocoele Fluid | 6 month | the presence of DNA will be evaluated through rtPCR |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Number of euploid samples | 8 months | Array CGH |
Countries
Italy
Outcome results
None listed