Lipid Metabolism
Conditions
Brief summary
A high-calcium intake can accelerate fat loss under energy-restricted diets. Part of this may be due to a shift in substrate metabolism where there is an increase in the rate of fat oxidation. However, whether high-calcium intake can influence substrate metabolism during exercise is not known. Accordingly, we aim to investigate the effect of 2 weeks of high-calcium intake on substrate metabolism during exercise.
Interventions
DIETARY_SUPPLEMENTCalcium
Milk-extracted calcium supplement will be used to modulate the calcium content of the milkshakes used.
DIETARY_SUPPLEMENTPlacebo
Sponsors
Northumbria University
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
DOUBLE (Subject, Investigator)
Eligibility
Sex/Gender
MALE
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* Physically active (moderate-vigorous exercise \>3 time per week) * Male * 18-40 yrs of age
Exclusion criteria
* Smoker * Known Food Allergies * Metabolic disorders
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Substrate metabolism | 60 s samples every 3 min for 15 min | Substrate metabolism will be assessed by the respiratory exchange ratio (rate of carbon dioxide production/rate of oxygen consumption) from samples of expired gas collected during the first 15 minutes of an incremental cycling test. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Plasma non-esterified fatty acid concentrations | Every 3 min for 15 min | Non-esterified fatty acid concentrations will be determined from plasma samples collected during the first 15 minutes of an incremental cycling test to indicate fatty acid availability. |
| Plasma glycerol concentrations | Every 3 min for 15 min | Glycerol concentrations will be determined from plasma samples collected during the first 15 minutes of an incremental cycling test as a marker of lipolysis. |
Other
| Measure | Time frame | Description |
|---|---|---|
| Serum parathyroid hormone concentration | Baseline (Pre-post 2 week supplementation) | Parathyroid hormone concentrations will be determined at rest before and after supplementation periods as a indicator of calcium status. |
| Plasma glucose-dependent insulinotropic peptide concentration | Baseline (Pre-post 2 week supplementation) | Glucose-dependent insulinotropic peptide concentrations will be determined at rest before and after supplementation periods as a potential mechanism of any changes in metabolism. |
| Plasma glucagon-like peptide-1 concentration | Baseline (Pre-post 2 week supplementation) | Glucagon-like peptide-1 concentrations will be determined at rest before and after supplementation periods as a potential mechanism of any changes in metabolism. |
Countries
United Kingdom
Outcome results
None listed