Exercise-induced Immune Dysfunction, Exercise-induced Inflammation, Exercise-induced Oxidative Stress
Conditions
Keywords
Inflammation, Oxidative stress, Immune dysfunction, Metabolomics.
Brief summary
Nutrasorb is a newly developed food product (see www.nutrasorb.com). This study will use the Nutrasorb soy protein product that is matrixed with polyphenols from blueberries and green tea extract, and test for efficacy as a nutritional countermeasure to exercise-induced physiologic stress (i.e., immune dysfunction, inflammation, and oxidative stress) using both traditional and metabolomics-based outcome measures.
Interventions
DIETARY_SUPPLEMENTNutrasorb
from blueberry and green tea extracts (2,136 mg/d gallic acid equivalents)
DIETARY_SUPPLEMENTPlacebo
Sponsors
Dole Food Company
Appalachian State University
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
DOUBLE (Subject, Investigator)
Eligibility
Sex/Gender
ALL
Age
18 Years to 55 Years
Healthy volunteers
Yes
Inclusion criteria
* Male or female endurance athletes; competitive runners or cyclists * Capable of exercising for 2.5 h at a high intensity in the lab * Ages 18-55 * Agree to train normally and stay weight stable. * Agree to avoid the use of large dose vitamin/mineral supplements * Agree to avoid herbs and medications that influence inflammation
Exclusion criteria
* Regularly take supplements or medicines known to effect inflammation. * At moderate or high risk for cardiovascular disease. * Younger than 18 or older than 55 years of age. * No history of competing in long distance running and cycling races.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Metabolomics; change in metabolites over time | 18-day period; Blood samples collected pre- and post-14 d supplementation, and immediately and 14 h after the third day of running | GC-MS and LC-MS through Metabolon |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Change in inflammation | 18-day period; Blood samples collected pre- and post-14 d supplementation, and immediately and 14 h after the third day of running | CRP and a cytokine panel |
| Change in oxidative stress | 18-day period; Blood samples collected pre- and post-14 d supplementation, and immediately and 14 h after the third day of running | F2-isoprostanes, protein carbonyls, FRAP, ORAC |
| Change in immune function | 18-day period; Blood samples collected pre- and post-14 d supplementation, and immediately and 14 h after the third day of running | Anti-viral activity using virus-infected HeLa cells |
Countries
United States
Outcome results
None listed