Arginine and Nitric Oxide (NO) Metabolism in Healthy Human Volunteers

Status

Completed

Phases

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT01774994

Enrollment

Registered

2013-01-24

Start date

2004-11-30

Completion date

2012-06-30

Last updated

2013-01-24

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Healthy

Keywords

volunteers

Brief summary

Investigation of arginine and NO metabolism in healthy subjects is important as a reference for alterations in arginine-NO metabolism in patients. Moreover, this study will indicate the changes during aging and gender-related differences. Although data on arginine metabolism exist in literature, data are limited to young healthy male subjects. Since patients with suspected changes in arginine metabolism, e.g. sepsis and cancer patients are often elderly and also women, knowledge of arginine metabolism in these groups is needed.

Interventions

OTHERstable isotope infusion for measurement of metabolism

Study design

Allocation

Intervention model

SINGLE_GROUP

Primary purpose

BASIC_SCIENCE

Eligibility

Sex/Gender

ALL

Age

18 Years to 70 Years

Healthy volunteers

Yes

Inclusion criteria

* Written informed consent * Age 18-25 years (young); 50-70 years (middle aged/elderly)

Exclusion criteria

* Diabetes mellitus type I * Livercirrhosis, pancreatitis, malignancies * Hypertension (diastolic blood pressure \>95 mmHg or systolic blood pressure \>160 mmHg) * History of gastrointestinal complaints * Recent involuntary weight loss (more than 10 % in 6 months or more than 5 % in one month) * Pregnancy (pregnancy test is done in female subjects) * Use of medication that can affect arginine metabolism (nitrovasodilators and corticosteroids) * Subjects with high activity level

Design outcomes

Primary

Measure	Time frame	Description
whole body NO synthesis	2 hours	postabsorptive nitric oxide synthesis measured by using stable isotope methodology

Secondary

Measure	Time frame	Description
whole body protein metabolism	2 hours	postabsorptive whole body protein metabolism (protein synthesis, breakdown, net protein breakdown) measured by using stable isotope methodology
whole body arginine metabolism	2 hours	postabsorptive arginine metabolism measured by using stable isotope methodology
blood parameters	2 hours	postabsorptive blood levels (glucose, insulin, urea, amino acids, CRP)

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026