Influence of Preconceptional Obesity on the Myometrial Cells Differentiation Toward a Contractyl Phenotype

Study of Role of Mononuclear Cells of Maternal Peripheric Blood in Human Myometrial Primar Cell Lines Differentiation Toward a Contratyl Phenotype: Influence of Preconceptional Obesity

Status

Completed

Phases

Unknown

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT01762683

Acronym

ObeDi

Enrollment

Registered

2013-01-08

Start date

2012-04-06

Completion date

2016-01-15

Last updated

2026-02-09

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Pregnancy Complication

Keywords

pregnancy, obesity, differentiation

Brief summary

The purpose of this study is to improve the understanding of cellular mecanism involved in delivery disorders in obese women, first by characterizing the hyperplasia phenomenon of myometrail cells induced by inflammatory stimulation (Il-1B), then by studying the role of blood mononuclear cells, of leptine and of obese women sera to interfere with the transition of myometrial cells toward hyperplasia phenotype.

Interventions

OTHERMyometrium biopsy and blood samples

The biopsy of myometrium is specially realised for the study in surgical unit after the release of the child, in women with normal pregnancy but necessitating a caesarean. The blood samples are realised, specially for the study, either during the medical exam before the ceasarean or specifically for the study.

Study design

Allocation

NON_RANDOMIZED

Intervention model

PARALLEL

Primary purpose

BASIC_SCIENCE

Masking

NONE

Eligibility

Sex/Gender

FEMALE

Age

18 Years to No maximum

Healthy volunteers

Yes

Inclusion criteria

* Informed consent signed * Patient affiliated with a social security scheme * In front of for obstetrical reasons to give birth by caesarean section (scheduled caesarean section) * Or a woman who is not obese in labour and needs to undergo a cesarean section * No obvious infectious context * Age \> 18 years old

Exclusion criteria

* Obvious inflammatory or autoimmune diseases * Suspected or confirmed infectious context, including virale infection VIH, VHC, VHB * Coagulation disorders

Design outcomes

Primary

Measure	Time frame	Description
Measuring the expression level of markers (Gama actine, Desmine, Connexine-43, and Ocytocine receptor) by Westernblotting	up to 2 months	Demonstrate in vitro the capacity of a Il-1B stimulus to induce the differentiation of the myometrial cells toward a hyperplasia phenotype, and that the answer depends on whether this is cells from normal weight women or obese women before conception.

Countries

France

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 10, 2026