Hepatitis C, Chronic, Interferon Deficiency, Liver Diseases
Conditions
Keywords
Hepatitis C Virus, HCV, Interferon, Prediction, SVR, Sustained Virus Response
Brief summary
The purpose of this study is to validate the first round HCV early dynamics discovery within a larger population.
Detailed description
Hepatitis C virus (HCV) infection rate in China is about 3%, which means about 30 million patients. Combination therapy of ribavirin and interferons (IFN) is the standard clinical treatment of HCV chronical infections. However, overall rate of sustained virological response (SVR) still do not exceed 60% even with ribavirin and peg-IFN. Due to several virus- and patient-related factors, treatment is even less successful in certain populations, especially in HCV genotype 1 infection. Thus the standard therapy duration is optimized according to the virus genotype in the clinical practice. Nowadays, two direct antiviral agents (DAAs) have been approved by Food and Drug Administration (FDA) of USA this year, which increases the SVR rate. However, high price, side effects and long duration render people to hesitate about the addition of the third drug in the traditional prescription. Predicting the outcome of traditional therapy is the cornerstone of the personalized therapy for HCV infected patients. In order to obtain an accurate prediction, different methods have been tried. Several indicators have been suggested to predict the final treatment outcomes. Rapid Virus Response (RVR), which indicates the non-detectable virus at the forth week since therapy starts, has been used to predict the final treatment outcome.Other indicators, including virus genotype, host genotype of IL-28B, human race and interferon stimulated genes (ISG) expression have also been shown to relate to and be able to predict the treatment outcomes to some extent. Here the investigators propose that the HCV virus dynamics analysis will give a more precise prediction for the therapy outcome. The general idea is that blood HCV titration data is obtained continuously in the early treatment period (first 2 weeks) of the patients who have strictly followed the therapy method. These titration data will be used to draw virus dynamics curve and calculate the corresponding parameters individually. The parameter(s) that can distinguish patients who reach the therapy evaluation standard from those who failed to reach the evaluation standard will be selected out, and such parameter(s) may be used to predict the therapy outcome of a new patient in the early stage of his/her treatment.
Interventions
Interferon:dosage,5 million units/person;frequency,every other day (qod);duration,48 weeks;Subcutaneous injection. Ribavirin: dosage,15mg/kg/day;frequency,three times a day (t.i.d);duration,48 weeks;take orally.
Sponsors
Chinese Academy of Sciences
Junqi Niu
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Healthy volunteers
No
Inclusion criteria
* Serologic evidence of chronic hepatitis C infection by an anti-HCV antibody test * Serum HCV-RNA \> 3 log IU/ml * Has been infected by HCV for more than 6 months * ALT,AST have been elevated continuously, inflammation and necrosis have been observed according to the histology diagnosis (G\>=2),modest liver fibrosis (S\>=2)For those patients whose ALT are normal,treatment accord to the liver biopsy. If obvious fibrosis has been detected (S2,S3),treatment should be done.For those S0,S1 stage patients, treatment could be delayed, but ALT/AST should be assayed every 3-6 months. * Compensated liver disease * Patients have never been treated with traditional interferon plus ribavirin or peginterferon plus ribavirin
Exclusion criteria
\- History: * Has history of decompensated liver diseases * Has been treated with other anti-virus drugs,or anti-tumor drugs,immuno-suppression drugs * Has a history of autoimmune hepatitis * History of a severe seizure disorder or current anticonvulsant use * History or other evidence of a medical condition associated with chronic liver disease other than HCV which would make the patient, in the opinion of the investigator, unsuitable for the study (e.g., hemochromatosis, autoimmune hepatitis, metabolic liver disease, alcoholic liver disease, toxin exposures) * Patients with documented or presumed coronary artery disease or cerebrovascular disease should not be enrolled if, in the judgment of the investigator, an acute decrease in hemoglobin by up to 4g/dL (as may be seen with ribavirin therapy) would not be well-tolerated * History of thyroid disease poorly controlled on prescribed medications, elevated thyroid stimulating hormone (TSH) concentrations with elevation of antibodies to thyroid peroxidase and any clinical manifestations of thyroid disease Current condition: * Pregnant women or women during the lactation period * Co-infected with hepatitis b virus or human immunodeficiency virus * Liver cancer or alpha-fetoprotein \> 100ng/ml * Blood neutrophils count \< 1500/mm3, or platelets count \< 90000/mm3 * Female hemoglobin \<11.5g/dL, male hemoglobin \<12.5g/dL * Blood creatinine \> 1.5 ULN * Have severe mental diseases,especially depression * Severe pulmonary dysfunction * Severe cardiovascular disease * Uncontrolled diabetes * Uncontrolled thalassemia * Evidence of alcohol abuse (alcohol consumption\>40 g/day) * Unwillingness to provide informed consent or abide by the requirements of the study * Local or System malignancy unstable status
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Absolute Blood HCV RNA Copies at designed time points | 0hr,24hr,1wk,2wk,4wk,6wk,12wk,24wk,48wk,72wk | Blood HCV RNA copies were assayed with Roche - COBAS® AmpliPrep/COBAS® TaqMan® HCV Test. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| HCV genotype | Baseline | HCV NS5A is cloned into T vector and sequenced for evolutionary analysis. |
| Alanine Aminotransferase (ALT) and Aspartate transaminase (AST) | Baseline,4wk,12wk,24wk,48wk | ALT AST are assayed to detect the hepatic function. |
| Fibrosis stage | Baseline,4wk,12wk,24wk,48wk | Fibrosis is analyzed with Fibroscan. |
| IL-28B polymorphism | Baseline | IL28 gene polymorphism,rs8099917,rs12979860,etc |
| Electrocardiography | Baseline,4wk,12wk,24wk,48wk | Electrocardiography is taken to avoid severe side effects. |
| Alcohol ,smoking condition | Baseline,4wk,12wk,24wk,48wk | Patients are asked whether they take alcohol or smoke cigarettes during the therapy period. |
| Drug abuse history | Baseline | Patients will be asked about their drug usage history. |
| Regular blood test | Baseline,4wk,12wk,24wk,48wk | The distribution and absolute count of the different types of blood cells are assayed. |
Countries
China
Contacts
Primary ContactChen Yang, PhD
Backup ContactXiumei Chi, PhD
Outcome results
None listed