Short Chain Fatty Acids Bioavailability
Conditions
Keywords
Short chain fatty acids, Acetate, Propionate, Butyrate, Bioavailability
Brief summary
The purpose of the study is to evaluate the bioavailability of acetate, propionate and butyrate in healthy humans using a stable isotope technology. In addition the level of acetate, propionate and butyrate production from inulin will be determined using the principle of isotope dilution.
Interventions
DIETARY_SUPPLEMENT13C-labeled Acetate
400 mg of sodium acetate 1-13C (2 colon delivery capsules with 200 mg)
DIETARY_SUPPLEMENT13C-labeled propionate
340 mg of sodium propionate 1-13C (2 colon delivery capsules with 170 mg)
DIETARY_SUPPLEMENT13C-labeled butyrate
990 mg sodium butyrate 1-13C (2 colon delivery capsules with 495 mg)
DIETARY_SUPPLEMENTInulin
15 g of inulin dissolved in 200 ml of water
Sponsors
KU Leuven
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Subject)
Eligibility
Sex/Gender
ALL
Age
18 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy volunteers * Regular dietary pattern (3 meals/day) * Age: 18-65y * BMI: 18,5-27 kg/m2
Exclusion criteria
* Intake of antibiotics 1 month prior to the study * Abdominal surgery in the past, with the exception of appendectomy * Intake of medication influencing the gastro-intestinal system 14 days prior to the study * In treatment at a dietician * Intake of pre- and/or probiotics * Exposure to radioactivity 1 year prior to the study * Serious chronic disease of the gastrointestinal tract * Use of medication that affects the gastro-intestinal tract during the last 2 weeks prior to the study * Pregnancy, pregnancy desire or lactation * Blood donation during the last 3 months prior to the study * Diabetes (type 1 or 2) * Aberrant hemoglobin level (Hb) in blood. Normal between 14.0 and 18.0 g/dL for men and between 12.0 and 16.0 g/dL for women
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Concentrations of the short chain fatty acids (acetate, propionate and butyrate) in plasma and urine samples | 12 hours each test day and 4 test days/volunteer | Participants performed each 4 test days and they were followed each test day for 12 hours, with sample collections at regular time points. Blood samples were collected every hour during the first 4 hours and afterwards every 20 minutes during 8 hours. Urine samples were collected during 24h in different fractions: 0-4h, 4-8h, 8-12h, 12-24h. |
Countries
Belgium
Outcome results
None listed