Systemic Inflammatory Response Syndrome
Conditions
Keywords
sepsis, cytokine release, fever
Brief summary
The investigators hypothesize that the investigators can detect protein-sparing effects of administration of the ketone 3-hydroxybutyrate and free fatty acids during simulation of an acute inflammatory disease. The investigators use the infusion of endotoxin, US standard reference E.coli in healthy subjects as a model for inflammation / infection and to evaluate the effect on protein metabolism using different tracers and the investigators can measure the various intracellular signaling pathways of selected muscle and adipose tissue.
Interventions
OTHEREndotoxin, US standard reference E.coli
Sponsors
University of Aarhus
Study design
Allocation
NON_RANDOMIZED
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Subject)
Eligibility
Sex/Gender
MALE
Age
20 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* 20 \< body mass index \< 30 kg/m2. * written and orally informed consent before enrollment.
Exclusion criteria
* participation in other studies using radioactive tracers or other exposure to radiation (X-rays, scintigraphy etc). * allergy to soy products or eggs * diabetes, any type * epilepsy * ongoing infection * immune deficiency * cardiovascular disease * dysregulated hypertension * primary muscles disease, congenital or acquired
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Protein metabolism | 6 hours intervention period | Measurements of protein oxidation during intervention using radioactive tracer techniques. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Activated intracellular signalling pathways | study day | Measurements on biopsies from muscle and fatty tissue taken on day of study |
Other
| Measure | Time frame |
|---|---|
| Other systemic metabolic effects | 6 hours of intervention |
Countries
Denmark
Outcome results
None listed