Metabolism
Conditions
Keywords
sugar sweetened beverages, fructose, lipogenesis, beta-oxidation
Brief summary
The objective of this study is to investigate the impact of sugar sweetened beverages on the fat metabolism of healthy young men. It is well known that consumption of beverages sweetened with fructose is associated with different health risks such as type 2 diabetes. The present study has been designed to dissect differences in the metabolic pathways of fructose and glucose, but also metabolic adaptations during fructose, glucose and sucrose diets. During a period of seven weeks subjects will consume either fructose, glucose or sucrose sweetened beverages or continue their usual drinking habits. During these seven weeks there will be different metabolic investigations using stable isotope tracers. First, the rate of lipolysis and beta-oxidation will be determined. Second, the rates of fatty acid synthesis will be measured. During all examinations there will also be substrate- and energy-utilization measurements by indirect calorimetry, blood analysis and morphometric measurements. Based on the literature main hypotheses are: Fructose enhances de novo lipogenesis postprandially and also in the fasting state significantly more than glucose by enhanced expression of lipogenic enzymes. Fructose decreases beta oxidation via downregulation of oxidative enzymes.
Interventions
PROCEDURESoft drink consumption
Sponsors
SNF Swiss National Foundation
University of Lausanne
University of Zurich
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
PREVENTION
Masking
QUADRUPLE (Subject, Caregiver, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
MALE
Age
18 Years to 30 Years
Healthy volunteers
No
Inclusion criteria
* Healthy male volunteers aged 18-30 * BMI between 19-24 kg/m2 * Non-smoker
Exclusion criteria
* Acute or chronic infections, malignant disease, renal, hepatic (more than two-fold increased transaminases), pulmonary, neurological (epilepsy) or psychiatric diseases, manifested atherosclerosis, or any other disease precluding participation in the study. * Diabetes * Known alcohol, substance or drug abuse, concomitant medication * More than three hours of physical exercise per week * Consumption of more than 2 times 3 dl SSB daily * Subjects likely to fail to comply with the study protocol * Subjects who do not give informed consent
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Lipogenesis | After total 6 weeks dietary intervention | Measurement of lipogenesis is based on i. v. administration of stable isotope labelled acetate (1,2-13C-acetate). 13C incorporation into palmitate is quantified by mass-spectrometry. 13C incorporation correlates to the rate of fatty acid synthesis. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Lipolysis | After total 6 weeks dietary intervention | Measurements using stable isotopes |
Other
| Measure | Time frame | Description |
|---|---|---|
| Waist/Hip Ratio | After total 6 weeks dietary intervention | Measurement waist/hip ratio using a nonstretchable band |
Countries
Switzerland
Outcome results
None listed