Sleep Deprivation, Sleep
Conditions
Brief summary
The study proposes to investigate whether sleep deprivation will affect a variety of measures, including hormones, immune system functioning, and behaviors related to food intake and hunger. It is predicted that sleep deprivation will affect circulating neutrophil activity, and do so via affects on DNA methylation. It is also predicted that sleep deprivation will up-regulate ghrelin, and down-regulate circulating oxytocin. Finally, it is predicted that sleep deprivation will increase participants' tendencies to pick larger portions of food, and also increase their tendency to purchase foods that are more caloric in a mock supermarket scenario.
Interventions
BEHAVIORALNeuroeconomics task
After a night of wakefulness, participants performed the neuroeconomics task (shopping food items in a mock supermarket scenario).
BEHAVIORALPortion Size Task
Participants are given a computer program that gives them the opportunity to choose the portions of a variety of food items that they would ideally like to consume
PROCEDUREBlood sample
After a night of wakefulness, a blood sample was taken to assess the level and efficacy of circulating neutrophils, as well as to assay blood serum and plasma for the presence of hormones involved in hunger such as oxytocin and ghrelin
Sponsors
Uppsala University
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
MALE
Healthy volunteers
Yes
Inclusion criteria
* Male * Age 18-35y * Healthy (self-reported) and not on medication * Non-smoking * Normal sleep-wake rhythm (i.e. 7-8 h per night, self-reported)
Exclusion criteria
* Major illness * Taking any serious medications * Any sleep conditions (e.g. irregular bedtimes, sleep complaints) * Any dietary issues with the food items provided * A history of endocrine or psychiatric disorders
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Neutrophil phagocytotic function production | Change in 4 neutrophil phagocytotic function from baseline to 36hours later (after the nighttime intervention) | This project is designed to test the inflammatory capacity of circulating neutrophils. It also aims to investigate if any changes in neutrophil efficacy are governed by alterations in DNA methylation. Measured during 36 hours (at 1930 day 1, 0730 and 1930 day 2, 0730 day 3) |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Portion Size Task | Change in selected portion size from baseline to 1 hr after consuming a caloric preload | Participants will be evaluated on their tendency to choose larger or smaller portions of a variety of meal items on a computer screen. This will be conducted both following sleep deprivation and sleep, and changes over the hour will be compared between these conditions. |
| Neuro-economics task | Change in purchasing behaviour 1 hr after consuming a caloric preload | Participants will be evaluated on their purchasing behavior with regards to high-calorie and low-calorie food items in a mock supermarket scenario following sleep and sleep deprivation. |
| Circulating hormone levels | Change in circulating hormone levels from baseline (ie. 1930 - before sleep intervention) to 12hr later (0730 after the nighttime intervention) | Participants will have their circulating hormone levels taken and analyzed, including ghrelin and oxytocin, to determine if sleep deprivation alters hormone levels related primarily to obesity or weight gain |
Countries
Sweden
Outcome results
None listed