Aging, Muscle Damage, Oxidative Stress
Conditions
Keywords
Aging, Skeletal muscle, Biomarkers, Adaptation
Brief summary
The purpose of the present investigation is to compare the responses and adaptations of young and elderly individuals to repeated eccentric exercise in regards to muscle function and redox homeostasis in a side-by-side comparison.
Detailed description
Despite the progress of analytic techniques and the refinement of study designs, striking disagreement exists among studies regarding the influence of exercise on muscle function and redox homeostasis in the elderly. A specific exercise protocol will be applied (the repeated eccentric model) to produce long-lasting and extensive changes in redox biomarkers and to examine more easily the potential effects of aging. Ten young men and ten elderly men will underwent an isokinetic eccentric exercise session, which will be repeated after three weeks. Muscle function indices and redox biomarkers will be assessed in urine, plasma or erythrocytes pre exercise, immediately post exercise, 2 and 4 days post exercise.
Interventions
OTHERExercise
Participants will perform two isokinetic eccentric exercise bouts
Sponsors
Aristotle University Of Thessaloniki
European University Cyprus
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
SUPPORTIVE_CARE
Masking
NONE
Eligibility
Sex/Gender
MALE
Age
18 Years to 70 Years
Healthy volunteers
Yes
Inclusion criteria
* subject provides written informed consent * physiological health profile * stable at their anthropometric characteristics for at least the last year
Exclusion criteria
* muscle disease * prior history of musculoskeletal injury to the lower limbs * smokers * consumed any nutritional supplement the last 3 months * performed intense eccentric exercise the last 6 months * non Caucasian
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Muscle torque (torque) | Change in muscle torque from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise |
| Range of motion, ROM (degrees) | Change in ROM from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise |
| Delayed onset muscle soreness, DOMS (scale 1-10) | Change in DOMS from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise |
| Creatine kinase, CK (activity IU) | Change in CK from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise |
Secondary
| Measure | Time frame |
|---|---|
| Superoxide dismutase, SOD (U/g Hb) | Change in SOD from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise |
| Glutathione peroxidase, GPx (U/g Hb) | Change in GPx from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise |
| Glucose-6-phosphate dehydrogenase, G6PD (U/g Hb) | Change in G6PD from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise |
| F2-isoprostanes (pg/mg creatinine) | Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise |
| Albumin (μM) | Change in albumin from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise |
| Bilirubin (μM) | Change in bilirubin from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise |
| Uric acid (mM) | Change in uric acid from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise |
| Protein carbonyls (nmol/mg protein) | Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise |
| Glutathione, GSH (μmol/g Hb) | Change in GSH from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise |
| Catalase (U/g Hb) | Change in catalase from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise |
Countries
Cyprus
Outcome results
None listed